Guinea pig Anti-P2X4 Receptor Antibody | Alomone Labs APR-002-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-P2X4 Receptor Antibody

catalog :

APR-002-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Olotu C, Kiefmann M, Ronneburg C, Lehmensiek F, Cuvenhaus A, Meidl V, et al. Analysis of purine receptor expression and functionality in alveolar epithelial cells. Purinergic Signal. 2020;16:213-229 pubmed publisher
Lalisse S, Hua J, Lenoir M, Linck N, Rassendren F, Ulmann L. Sensory neuronal P2RX4 receptors controls BDNF signaling in inflammatory pain. Sci Rep. 2018;8:964 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 4), mouse brain (lanes 2 and 5) and human U-87 MG glioblastoma cell (lanes 3 and 6) lysates: - 1,2. Guinea pig Anti-P2X4 Receptor Antibody (#APR-002-GP), (1:500).3. Guinea pig Anti-P2X4 Receptor Antibody (1:1000).4-6. Guinea pig Anti-P2X4 Receptor Antibody, preincubated with P2X4 Receptor Blocking Peptide (#BLP-PR002).

product information

CAT :

APR-002-GP

SKU :

APR-002-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-P2X4 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P51577

Applications :

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PR002

Homology :

Mouse - identical; human - 15/19 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KKYKYVEDYEQGLSGEMNQ, corresponding to amino acid residues 370-388 of rat P2X4 receptor

Product Page - Scientific background :

The P2X purinergic receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.The structure and function of the P2X receptors, which were mainly investigated using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.Seven mammalian P2X receptor subtypes (P2X1-P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N- and C-termini, two membrane-spanning domains and a large extracellular loop.All P2X subunits can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only appears to function as part of a heteromeric complex.4-9The various P2X receptors show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4The P2X2 receptor subunit has a widespread tissue distribution in autonomic neurons, but it is generally found to be co-expressed with one or more subtypes.Overexpression of P2X4 was demonstrated in microglia and in the spinal dorsal horn following peripheral nerve injury. It has been suggested that activation of P2X4 along with p38 MAPK is essential for the development of allodynia (pain from a stimulus that doesn't normally elicit pain) following nerve injury. Inhibition of P2X4 expression in spinal microglia has been suggested as a novel therapeutic approach for the treatment of allodynia.10

Applications may also work in :

Supplier :

Alomone Labs

Target :

P2RX4, P2X purinoceptor 4, ATP receptor

Short Description :

A Guinea Pig Polyclonal Antibody to P2X4 Receptor

Long Description :

Guinea pig Anti-P2X4 Receptor Antibody (#APR-002-GP, formerly AGP-085), raised in guinea pigs, is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. It has been designed to recognize P2X4 from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-P2X4 Receptor Antibody (#APR-002) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-PR002

Positive Control :

Synonyms :

P2RX4, P2X purinoceptor 4, ATP receptor

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500-1:1000

more info or order :

Alomone Labs product webpage