Anti-P2X1 Receptor Antibody | Alomone Labs APR-001 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-P2X1 Receptor Antibody

catalog :

APR-001

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 17

Reference
Wang X, Yuan X, Su Y, Hu J, Ji Q, Fu S, et al. Targeting Purinergic Receptor P2RX1 Modulates Intestinal Microbiota and Alleviates Inflammation in Colitis. Front Immunol. 2021;12:696766 pubmed publisher
Hong S, Yang M, Cho H, Park I, Bae H, Choe K, et al. Distinct fibroblast subsets regulate lacteal integrity through YAP/TAZ-induced VEGF-C in intestinal villi. Nat Commun. 2020;11:4102 pubmed publisher
Kim M, Yu H, Ju H, Shin J, Kim A, Lee J, et al. Induction of detrusor underactivity by extensive vascular endothelial damages of iliac arteries in a rat model and its pathophysiology in the genetic levels. Sci Rep. 2019;9:16328 pubmed publisher
Carluccio M, Zuccarini M, Ziberi S, Giuliani P, Morabito C, Mariggio M, et al. Involvement of P2X7 Receptors in the Osteogenic Differentiation of Mesenchymal Stromal/Stem Cells Derived from Human Subcutaneous Adipose Tissue. Stem Cell Rev. 2019;: pubmed publisher
Chavez J, Vargas M, Martínez Zúñiga J, Falfan Valencia R, Ambrocio Ortiz E, Carbajal V, et al. Allergic sensitization increases the amount of extracellular ATP hydrolyzed by guinea pig leukocytes. Purinergic Signal. 2019;15:69-76 pubmed publisher
Fryatt A, Dayl S, Stavrou A, Schmid R, Evans R. Organization of ATP-gated P2X1 receptor intracellular termini in apo and desensitized states. J Gen Physiol. 2019;151:146-155 pubmed publisher
Huo H, Fryatt A, Farmer L, Schmid R, Evans R. Mapping the binding site of the P2X receptor antagonist PPADS reveals the importance of orthosteric site charge and the cysteine-rich head region. J Biol Chem. 2018;293:12820-12831 pubmed publisher
Ji R, Zhu J, Wang D, Sui Q, Knight G, Burnstock G, et al. Expression of P2X1 receptors in somatostatin-containing cells in mouse gastrointestinal tract and pancreatic islets of both mouse and human. Purinergic Signal. 2018;14:285-298 pubmed publisher
Yu W, Hill W, Robson S, Zeidel M. Role of P2X4 Receptor in Mouse Voiding Function. Sci Rep. 2018;8:1838 pubmed publisher
Koessler J, Schwarz M, Weber K, Etzel J, Koessler A, Böeck M, et al. The role of adenosine diphosphate mediated platelet responsiveness for the stability of platelet integrity in citrated whole blood under ex vivo conditions. PLoS ONE. 2017;12:e0188193 pubmed publisher
Odom M, Pak E, Brown D, Hannan J. Enhanced Electrical Field Stimulated Nitrergic and Purinergic Vasoreactivity in Distal vs Proximal Internal Pudendal Arteries. J Sex Med. 2017;14:1285-1296 pubmed publisher
Teng B, Labazi H, Sun C, Yang Y, Zeng X, Mustafa S, et al. Divergent coronary flow responses to uridine adenosine tetraphosphate in atherosclerotic ApoE knockout mice. Purinergic Signal. 2017;13:591-600 pubmed publisher
Ilkan Z, Wright J, Goodall A, Gibbins J, Jones C, Mahaut Smith M. Evidence for shear-mediated Ca2+ entry through mechanosensitive cation channels in human platelets and a megakaryocytic cell line. J Biol Chem. 2017;292:9204-9217 pubmed publisher
Sander P, Mostafa H, Soboh A, Schneider J, Pala A, Baron A, et al. Vacquinol-1 inducible cell death in glioblastoma multiforme is counter regulated by TRPM7 activity induced by exogenous ATP. Oncotarget. 2017;8:35124-35137 pubmed publisher
Reigada D, Navarro Ruiz R, Caballero López M, Del Águila A, Muñoz Galdeano T, Maza R, et al. Diadenosine tetraphosphate (Ap4A) inhibits ATP-induced excitotoxicity: a neuroprotective strategy for traumatic spinal cord injury treatment. Purinergic Signal. 2017;13:75-87 pubmed publisher
Wright A, Mahaut Smith M, Symon F, Sylvius N, Ran S, Bafadhel M, et al. Impaired P2X1 Receptor-Mediated Adhesion in Eosinophils from Asthmatic Patients. J Immunol. 2016;196:4877-84 pubmed publisher
Seref Ferlengez Z, Maung S, Schaffler M, Spray D, Suadicani S, Thi M. P2X7R-Panx1 Complex Impairs Bone Mechanosignaling under High Glucose Levels Associated with Type-1 Diabetes. PLoS ONE. 2016;11:e0155107 pubmed publisher

image

image 1 :

Western blot analysis of human platelet lysates: - 1. Anti-P2X1 Receptor Antibody (#APR-001), (1:200).2. Anti-P2X1 Receptor Antibody, preincubated with P2X1 Receptor Blocking Peptide (#BLP-PR001).

image 2 :

Expression of P2X1 Receptor in rat hypothalamus - Immunohistochemical staining of rat supra-optic nucleus using Anti-P2X1 Receptor Antibody (#APR-001). P2X1 receptor (green) appears in axonal processes (vertical arrows) and cell bodies (horizontal arrows). DAPI is used as the counterstain (blue).

image 3 :

Expression of P2X1 Receptor in rat hypothalamus - Immunohistochemical staining of rat supra-optic nucleus usingAnti-P2X1 ReceptorAntibody (#APR-001). P2X1 receptor (green) appears in axonal processes (vertical arrows) and cell bodies (horizontal arrows). DAPI is used as the counterstain (blue).

product information

CAT :

APR-001

SKU :

APR-001-CF_0.2 ml

Product Name :

Anti-P2X1 Receptor Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P47824

Applications :

IC IF IFC IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PR001

Homology :

Mouse - 17/18 amino acid residues identical; human - 15/18 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(CS)DPVATSSTLGLQENMRTS, corresponding to amino acid residues 382-399 of rat P2X1 receptor

Product Page - Scientific background :

The P2X receptors belong to the ligand-gated ion channel family and are activated by extracellular ATP.The structure and function of the P2X receptors, investigated mainly using in vitro models, indicate their involvement in synaptic communication, cell death, and differentiation.Seven mammalian P2X receptor subtypes (P2X1-P2X7) have been identified and cloned.1,2,3 All P2X receptor subtypes share the same structure of intracellular N and C-termini two membrane-spanning domains and a large extracellular loop.All P2X receptor subtypes can assemble to form homomeric or heteromeric functional channels with the exception of P2X6, which only seems to function as part of a heteromeric complex.4-9The various P2X receptor subtypes show distinct expression patterns. P2X1-6 have been found in the central and peripheral nervous system, while the P2X7 receptor is predominantly found in cells of the immune system.4 The P2X1 receptor is present in smooth muscle, cerebellum, dorsal horn spinal neurons, and platelets where it is suggested to play a regulatory role during in vivo homeostasis and thrombosis.3,4,10,11

Applications may also work in :

IC IF IFC IHC IP WB

Supplier :

Alomone Labs

Target :

P2RX1, P2X purinoceptor 1

Short Description :

A Rabbit Polyclonal Antibody to P2X1 Receptor

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat P2X1 receptor. Anti-P2X1 Receptor Antibody (#APR-001) can be used in western blot, immunoprecipitation, indirect flow cytometry, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize P2X1 receptor from rat, mouse, and human samples.

Negative Control :

BLP-PR001

Positive Control :

Synonyms :

P2RX1, P2X purinoceptor 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

P2RX1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage