antibody

Anti-TMEM175 Antibody

APC-175

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry

APC-175-CF_0.2 ml

Anti-TMEM175 Antibody

Antibodies

Antibodies

Polyclonal

Q9CXY1

IHC WB

Human Rat Mouse

Rabbit

BLP-PC175

Rat - 13 out of 14 amino acid residues identical Human - 12 out of 14 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Luminal, 4th loop.

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Mouse

CEDNVPDPKDVQEK, corresponding to amino acid residues 278 - 291 of mouse TMEM175

Transmembrane protein 175, TMEM175, is an endosomal/lysosomal proton channel that plays a crucial role in preserving the lysosomal membrane potential and maintaining pH stability. TMEM175 mediates lysosomal proton efflux, known as the "H+ leak" pathway, preventing excessive acidification of lysosomes.1 Intracellular endosomes and lysosomes rely on a vacuolar-type H+ ATPase (V-ATPase) to establish a significant proton concentration gradient across their membranes, which plays a crucial role in facilitating active transport of ions and metabolites between the vesicular lumen and the cytosol. To effectively digest macromolecules, lysosomes rely on an acidic environment with a pH range of 4.5 to 5.0, which is maintained through the entry of protons by the V-ATPase pump and their exit through the "H+ leak" pathway, which is the passive release of H+ into the cytosol. TMEM175 functions as a proton-activated, proton-selective channel on the lysosomal membrane in order to mediate the "H+ leak" pathway, prohibiting the excessive acidification of lysosomes. When lysosomes are acidified beyond the typical range, TMEM175 is activated, prohibiting further lysosomal acidification.1Mammalian TMEM175 consists of two homologous 6-TM domains and is suggested to function as a dimer. TM1 serves as the inner helix lining the pore and forms an hourglass-shaped ion permeation pathway within the channel. The C-terminal half of TM1 contains hydrophobic residues that create a bottleneck along the ion conduction pathway, serving as the selectivity filter of the channel. TMEM175 conducts K+ constitutively at pH 7.4, but shows reduced K+ permeation at lower pH. Conversely, proton current through TMEM175 increases with decreasing pH due to the increased proton gradient. Molecular dynamics simulation, structure-based mutagenesis, and electrophysiological analysis suggest that K+ ions and protons share the same permeation pathway in TMEM175.2,3Altered lysosomal pH disrupts various cellular processes, including lysosomal degradation, cargo loading, catabolite export, vesicle movement, and nutrient sensing. This impairment contributes to the pathogenesis of neurodegenerative diseases such as Parkinson's disease, Alzheimer's disease, and lysosomal storage diseases. Optimization of lysosomal pH can effectively clear toxic protein aggregates associated with these diseases. Thus, maintaining lysosomal pH homeostasis plays a crucial role in lysosome function, cellular health, and neuropathology.1

IHC WB

Alomone Labs

Transmembrane Protein 175, Endosomal/Lysosomal Proton Channel TMEM175

A Rabbit Polyclonal antibody to TMEM175

Anti-TMEM175 Antibody (#APC-175) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize TMEM175 from rat, mouse and human samples.

BLP-PC175

NA

Transmembrane Protein 175, Endosomal/Lysosomal Proton Channel TMEM175

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

TMEM175

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:300

1:200