antibody

Anti-KCNK16 (TALK-1) (extracellular) Antibody

APC-170

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry

APC-170-CF_0.2 ml

Anti-KCNK16 (TALK-1) (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q96T55

IHC WB

Human Rat Mouse

Rabbit

BLP-PC170

Rat, mouse - identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

1st extracellular loop

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Human

(C)EAWVKGVNPKGNST, corresponding to amino acid residues 75-88 of human KCNK16

The KCNK16 gene encodes the TALK-1 channel, an abundant K+ channel transcript found in mouse and in human β-cells. There are four human TALK-1 transcript variants, including two that form functional K+ channels (TALK-1a (transcript variant 2) and TALK1-b (transcript variant 3)1,2. The TALK-1 structure includes 4 transmembrane domains, 2 extracellular loops, and intracellular N- and C-termini. The C-terminal tail of TALK-1 is a critical component that influences the magnitude of TALK-1 channel activation, while the N-terminus is important for protein interaction1,2.TALK-1, two-pore domain K+ (K2P16.1) channel is responsible for modulating glucose stimulated insulin secretion that relies on β-cell Ca2+ influx. The channel produces outwardly rectifying, non-inactivating K+ currents, which are enhanced by elevations in extracellular pH. TALK-1 is thus pH-sensitive, with increased activity under alkaline conditions. In addition, it can be activated by singlet oxygen and nitric oxide.A gain-of-function polymorphism in the KCNK16 gene is associated with increased risk for developing type-2 diabetes1,2.

IHC WB

Alomone Labs

Potassium two pore domain channel subfamily K member 16, TWIK-related alkaline pH-activated K+ channel 1, K2P16.1

A Rabbit Polyclonal Antibody to KCNK16 Channel

Anti-KCNK16 (TALK-1) (extracellular) Antibody (#APC-170) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, analysis. The antibody recognizes an extracellular epitope, and can potentially be used for detecting the protein in living cells. It has been designed to recognize TALK-1 from rat, mouse, and human samples.

BLP-PC170

NA

Potassium two pore domain channel subfamily K member 16, TWIK-related alkaline pH-activated K+ channel 1, K2P16.1

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

KCNK16

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

yes

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:150

1:600