antibody

Anti-KCNK12 (THIK-2) (extracellular) Antibody

APC-169

polyclonal

domestic rabbit

nonconjugated

NA

human, mouse, rat

western blot, immunohistochemistry, immunocytochemistry

APC-169-CF_0.2 ml

Anti-KCNK12 (THIK-2) (extracellular) Antibody

Antibodies

Antibodies

Polyclonal

Q9ERS1

IF IHC WB

Human Rat Mouse

Rabbit

BLP-PC169

Mouse, human - identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

1st extracellular loop

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)ESPGEAEARARWG, corresponding to amino acid residues 61-73 of rat KCNK12

Potassium channels form the largest ion channel family with over 70 genes identified in humans. 2-pore domain channels (K2P) are believed to be responsible for background or leak K+ currents and they are widely expressed in the nervous system. They are involved in resting potential control and firing pattern of excitable cells1. The K2P channel family is comprised of at least 16 members.The KCNK12 channel (THIK-2, K2P12.1) has two pore domains (M1 and P1) and a long M1-P1 linker. It has a long cytosolic C terminus and a very unusual N-terminal domain containing many cysteine, proline and arginine residues. This N-terminal domain cannot be found in the similar THIK-1 channel and is highly conserved in mammals.Until recently, the THIK-2 channel was considered to be a "silent" channel and could not be functionally expressed in mammalian and non-mammalian cell lines. When removing the N-terminal both THIK-1 and THIK-2 channels exhibit similar current-voltage relations. In addition, both channels have 64% identical amino-acids in humans and 59% in rats. Only a small fraction of THIK-2 channel is localized to the cell membrane surface and this can be increased by deleting the original retention/retrieval signal RRR in its N-terminus.The THIK-2 channel is highly expressed in the cortex, cerebellum hippocampus, thalamus, inferior colliculi of the midbrain and the olfactory bulb. It is also expressed in non-neuronal tissues such as the lungs, liver, kidney and spleen2.

IF IHC WB

Alomone Labs

Potassium channel subfamily K member 12, Tandem pore domain halothane-inhibited K+ channel 2, K2P12.1

A Rabbit Polyclonal Antibody to KCNK12 Channel

Anti-KCNK12 (THIK-2) (extracellular) Antibody (#APC-169) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KCNK12 from rat and mouse and human samples.

BLP-PC169

NA

Potassium channel subfamily K member 12, Tandem pore domain halothane-inhibited K+ channel 2, K2P12.1

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

KCNK12

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

yes

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:400

1:200-1:400