Anti-KCNQ1 (extracellular)-FITC Antibody | Alomone Labs APC-168-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNQ1 (extracellular)-FITC Antibody

catalog :

APC-168-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

flow cytometry

more info or order :

Alomone Labs product webpage

image

image 1 :

Cell surface detection of KCNQ1 by direct flow cytometry in live intact mouse J774 macrophage cells: Cell surface detection of KCNQ1 by direct flow cytometry in live intact mouse J774 macrophage cells: Black line: Cells. Red line: Cells + Rabbit IgG Isotype Control-FITC (#RIC-001-F). Green line: Cells + Anti-KCNQ1 (extracellular)-FITC Antibody (#APC-168-F), (2.5µg).

product information

CAT :

APC-168-F

SKU :

APC-168-F_15 mcl

Product Name :

Anti-KCNQ1 (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Z0N7

Applications :

FC LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC168

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

3rd extracellular loop

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EKDAVNESGRIEFG, corresponding to amino acid residues 284-297 of rat KCNQ1

Product Page - Scientific background :

KCNQ1 (KV7.1) is part of a voltage-gated K+ family that includes 5 other members (KCNQ1-5). The channel has been intensively studied since it was found that together with MinK (Isk) it underlay the cardiac Iks current that controls the duration of the action potential of the human heart. Indeed mutations on either KV7.1 or MinK can be responsible for long QT syndrome, a cardiac disorder that causes arrhythmias and sudden death. Although KV7.1 expression and function has been mainly investigated in relation with cardiac function the channel is widely distributed in epithelial tissues. There it has been implicated in physiological functions such as: regulation of acid secretion in the stomach and Cl- secretion into the colon.

Applications may also work in :

FC LCI

Supplier :

Alomone Labs

Target :

KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KvLQT1, KCNA9

Short Description :

A Rabbit Polyclonal Antibody to KV7.1 (extracellular) conjugated to the fluorescent dye FITC

Long Description :

Anti-KCNQ1 (extracellular) Antibody (#APC-168) is a highly specific antibody directed against an extracellular epitope of the rat KCNQ1 (KV7.1). The antibody can be used in western blot, immunocytochemistry and live cell flow cytometry applications. It has been designed to recognize KCNQ1 from mouse, rat and human samples. Anti-KCNQ1 (extracellular)-FITC Antibody (#APC-168-F) is directly conjugated to fluorescein isothiocyanate (FITC) fluorophore. This conjugated antibody has been developed to be used in immunofluorescent applications such as direct flow cytometry and live cell imaging. It has been designed to recognize KCNQ1 from mouse, rat and human samples.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

KV7.1, Voltage-gated potassium channel QKT subfamily member 1, KvLQT1, KCNA9

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNQ1

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APC-168), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage