Anti-KV1.4 (extracellular) Antibody | Alomone Labs APC-167 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV1.4 (extracellular) Antibody

catalog :

APC-167

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Alvarez P, Bogen O, Levine J. Interleukin 6 decreases nociceptor expression of the potassium channel KV1.4 in a rat model of hand-arm vibration syndrome. Pain. 2019;160:1876-1882 pubmed publisher
Vivekananda U, Novak P, Bello O, Korchev Y, Krishnakumar S, Volynski K, et al. Kv1.1 channelopathy abolishes presynaptic spike width modulation by subthreshold somatic depolarization. Proc Natl Acad Sci U S A. 2017;114:2395-2400 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:400).2. Anti-KV1.4 (extracellular) Antibody, preincubated with Kv1.4 (extracellular) Blocking Peptide (#BLP-PC167).

image 2 :

Expression of KV1.4 in rat parietal cortex - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-KV1.4 (extracellular) Antibody (#APC-167), (1:400), followed by donkey-anti-rabbit-biotin and streptavidin-Cy3. KV1.4 staining (red) appears in several neuronal-outlined cells (arrows). Nuclei were stained with DAPI (blue).

product information

CAT :

APC-167

SKU :

APC-167-CF_0.2 ml

Product Name :

Anti-KV1.4 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P15385

Applications :

IC IF IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC167

Homology :

Mouse - identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)GHSRLLNDTSAPHLE, corresponding to amino acid residues 348 - 362 of rat KV1.4

Product Page - Scientific background :

KV1.4 is a mammalian voltage-dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.4 was first cloned from rat brain.1 Eight Shaker-related genes exist in mammals constituting the KV1 subfamily of the large KV channel family of genes.2A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition, several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.The structure of KV1.4 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2The channel is expressed in neurons and cardiac and skeletal muscle tissue as well as in the pancreas.2 The functional channel is considered transient (A-type) current and shows pronounced inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.KV1.4 channels are sensitive to high doses of TEA (>100 mM) and low doses of 4-AP (0.013 mM), the "classical" non-selective potassium channel blockers.Most venomous peptide toxins that affect other KV channels do not inhibit KV1.4. However, the sea anemone toxin Stichodactyla Toxin (ShK), which is more potent towards KV1.1 and KV1.3, is still a potent inhibitor of KV1.4 channels.3

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily A member 4, KCNA4

Short Description :

A Rabbit Polyclonal Antibody to KV1.4 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KV1.4. Anti-KV1.4 (extracellular) Antibody (#APC-167) can be used in western blot, immunohistochemistry and live cell imaging applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize KV1.4 from human, rat and mouse samples.

Negative Control :

BLP-PC167

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily A member 4, KCNA4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNA4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage