Anti-Kir4.1 (KCNJ10) (extracellular) Antibody | Alomone Labs APC-165 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Kir4.1 (KCNJ10) (extracellular) Antibody

catalog :

APC-165

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Gilbert A, Elorza Vidal X, Rancillac A, Chagnot A, Yetim M, Hingot V, et al. Megalencephalic leukoencephalopathy with subcortical cysts is a developmental disorder of the gliovascular unit. elife. 2021;10: pubmed publisher
Huang B, Zdora I, de Buhr N, Lehmbecker A, Baumg xe4 rtner W, Leitzen E. Phenotypical peculiarities and species-specific differences of canine and murine satellite glial cells of spinal ganglia. J Cell Mol Med. 2021;25:6909-6924 pubmed publisher
Schirmer L, Mobius W, Zhao C, Cruz Herranz A, Ben Haim L, Cordano C, et al. Oligodendrocyte-encoded Kir4.1 function is required for axonal integrity. elife. 2018;7: pubmed publisher
Kelley K, Ben Haim L, Schirmer L, Tyzack G, Tolman M, Miller J, et al. Kir4.1-Dependent Astrocyte-Fast Motor Neuron Interactions Are Required for Peak Strength. Neuron. 2018;98:306-319.e7 pubmed publisher
Cui Y, Yang Y, Ni Z, Dong Y, Cai G, Foncelle A, et al. Astroglial Kir4.1 in the lateral habenula drives neuronal bursts in depression. Nature. 2018;554:323-327 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysates (lanes 1 and 3) and mouse brain lysates (lanes 2 and 4): - 1, 2. Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165), (1:500).3, 4. Anti-Kir4.1 (KCNJ10) (extracellular) Antibody, preincubated with Kir4.1/KCNJ10 (extracellular) Blocking Peptide (#BLP-PC165).

image 2 :

Peptide (C)HGDLLELGPPANHT corresponding to amino acid residues 93-106 of rat Kir4.1 (AccessionP49655). Extracellular loop.

image 3 :

Expression of Kir4.1 in human U-87 MG cells - Cell surface detection ofKir4.1 in live intact human glioblastoma U-87 MG cells. A. Extracellular staining of cells usingAnti-Kir4.1(KCNJ10)(extracellular) Antibody (#APC-165) (1:100) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live image of the cells. C. Merge of A and B.

product information

CAT :

APC-165

SKU :

APC-165-CF_0.2 ml

Product Name :

Anti-Kir4.1 (KCNJ10) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P49655

Applications :

IC IF IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC165

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)HGDLLELGPPANHT, corresponding to amino acid residues 93-106 of rat Kir4.1

Product Page - Scientific background :

Kir4.1 is a member of the inward rectifying K+ channel family. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunit that can assembly as either homo or heteromers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.Kir4.1 is a member of the Kir4 subfamily that includes one other member: Kir4.2. Kir4.1 can co-assemble with Kir4.2 but also with other Kir channels such as Kir2.1 and Kir5.1.The Kir4 subfamily has been classified as weak rectifiers with intermediate conductance. Kir4.1, encoded by KCNJ10, is mainly expressed in brain, specifically in glia cells, but also in retina, ear and kidney.1,2It has been proposed that Kir4.1 has an essential role in glial K+ buffering, a process that re-uptakes the K+ released during neuronal activity into the intracellular interstitial space. Loss of Kir4.1 causes retinal defects and loss of endochoclear potential.3

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10

Short Description :

A Rabbit Polyclonal Antibody to Kir4.1 (KCNJ10) Channel

Long Description :

Anti-Kir4.1 (KCNJ10) (extracellular) Antibody (#APC-165) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and live cell imaging applications. It has been designed to recognize Kir4.1 potassium channel from rat, mouse, and human samples.

Negative Control :

BLP-PC165

Positive Control :

Synonyms :

ATP-sensitive inward rectifier potassium channel 10, KAB-2, BIR10, BIRK1, Kir1.2, Potassium channel inwardly rectifying subfamily J member 10

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ10

AB Specifictiy :

May have some cross-reactivity with rat Kir4.2 (Accession Q91ZF1).

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage