Anti-KCNQ4 Antibody | Alomone Labs APC-164 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNQ4 Antibody

catalog :

APC-164

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Perez Flores M, Lee J, Park S, Zhang X, Sihn C, Ledford H, et al. Cooperativity of Kv7.4 channels confers ultrafast electromechanical sensitivity and emergent properties in cochlear outer hair cells. Sci Adv. 2020;6:eaba1104 pubmed publisher
Inagaki A, Hayashi M, Andharia N, Matsuda H. Involvement of butyrate in electrogenic K+ secretion in rat rectal colon. Pflugers Arch. 2019;471:313-327 pubmed publisher
Paz R, Tubert C, Stahl A, Díaz A, Etchenique R, Murer M, et al. Inhibition of striatal cholinergic interneuron activity by the Kv7 opener retigabine and the nonsteroidal anti-inflammatory drug diclofenac. Neuropharmacology. 2018;137:309-321 pubmed publisher
Li L, Sun H, Ding J, Niu C, Su M, Zhang L, et al. Selective targeting of M-type potassium Kv 7.4 channels demonstrates their key role in the regulation of dopaminergic neuronal excitability and depression-like behaviour. Br J Pharmacol. 2017;174:4277-4294 pubmed publisher
Goodwill A, Fu L, Noblet J, Casalini E, Sassoon D, Berwick Z, et al. KV7 channels contribute to paracrine, but not metabolic or ischemic, regulation of coronary vascular reactivity in swine. Am J Physiol Heart Circ Physiol. 2016;310:H693-704 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-KCNQ4 Antibody (#APC-164), (1:200).3,4. Anti-KCNQ4 Antibody, preincubated with KCNQ4 Blocking Peptide (#BLP-PC164).

image 2 :

Expression of KCNQ4 (KV7.4) in rat lateral dorsal raphe nucleus - Immunohistochemical staining of perfusion-fixed frozen rat brain sections using Anti-KCNQ4 Antibody (#APC-164), (1:400), followed by biotin-labeled anti-rabbit antibody and streptavidin-ATTO Fluor-594 (red). Staining appears in soma of neuronal profiles (arrows). Nuclei are stained with DAPI (blue).

product information

CAT :

APC-164

SKU :

APC-164-CF_0.2 ml

Product Name :

Anti-KCNQ4 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JK97

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC164

Homology :

Rat - identical; human - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)DRKTREKGDKGPSD, corresponding to amino acid residues 594-607 of mouse KCNQ4

Product Page - Scientific background :

The KCNQ (KV7) family of channels consists of five members (KCNQ1, KCNQ2, KCNQ3, KCNQ4 and KCNQ5). KCNQ channels commonly display activation at voltages close to neuronal resting membrane potentials and are regulated by G-protein coupled receptor (GPCR) signaling, notably by muscarinic receptors1.The KCNQ4 (KV7.4) encodes a protein of 695 amino acid residues. The homology with other members of the KCNQ family ranges from 37 to 44% at the amino acid level. KCNQ4 protein contains six domains that span the plasma membrane, and a P-loop domain which forms the K+-selective channel pore. The fourth transmembrane domain contains the voltage sensor, which is responsible for the electrically driven conformational change that leads to channel opening. Functional channels are formed by a tetrameric assembly of KCNQ4 subunits, typically in homotetrameric form, but heterotetrameric co-assembly of different members is allowed2.KCNQ2-KCNQ5 are predominantly expressed in the peripheral and central nervous system, including hippocampal cells, cortical cells, and dorsal root ganglion3. In addition KCNQ4 is expressed in the heart and in the outer, but not in the inner, sensory hair cells of the cochlea4.It has been demonstrated that KCNQ4 mutations may be a relatively frequent cause of autosomal dominant hearing loss4.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

KV7.4, Potassium voltage-gated channel subfamily KQT member 4, KQT-like 4, KvLQT4, DFNA2

Short Description :

A Rabbit Polyclonal Antibody to KCNQ4 (KV7.4) Channel

Long Description :

Anti-KCNQ4 Antibody (#APC-164) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV7.4 from rat, human, and mouse samples.

Negative Control :

BLP-PC164

Positive Control :

Synonyms :

KV7.4, Potassium voltage-gated channel subfamily KQT member 4, KQT-like 4, KvLQT4, DFNA2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNQ4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage