Anti-KCNE1 (IsK) Antibody | Alomone Labs APC-163 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNE1 (IsK) Antibody

catalog :

APC-163

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Cechmanek P, Hehr C, McFarlane S. Retinal Pigment Epithelium and Neural Retinal Progenitors Interact via Semaphorin 6D to Facilitate Optic Cup Morphogenesis. Eneuro. 2021;8: pubmed publisher
Strigli A, Raab C, Hessler S, Huth T, Schuldt A, Alzheimer C, et al. Doxorubicin induces caspase-mediated proteolysis of KV7.1. Commun Biol. 2018;1:155 pubmed publisher
Lv Y, Wang Y, Zhu X, Zhang H. Aldosterone downregulates delayed rectifier potassium currents through an angiotensin type 1 receptor-dependent mechanism. Am J Transl Res. 2018;10:1413-1421 pubmed

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 4), rat heart (lanes 2 and 5) and mouse kidney (lanes 3 and 6) membranes: - 1-3. Anti-KCNE1 (IsK) Antibody (#APC-163), (1:200).4-6. Anti-KCNE1 (IsK) Antibody, preincubated with KCNE1/IsK Blocking Peptide (#BLP-PC163).

image 2 :

Western blot analysis of rat kidney (lanes 1 and 4) rat heart (lanes 2 and 5) and mouse kidney (lanes 3 and 6) membranes: - 1-3.Anti-KCNE1 (IsK) Antibody (#APC-163) (1:200).4-6. Anti-KCNE1 (IsK) Antibody preincubated with the negative control antigen.

image 3 :

Western blot analysis of human SH-SY5Y neuroblastoma (lanes 1 and 4) human HT-29 colon adenocarcinoma (lanes 2 and 5) and human MCF-7 breast adenocarcinoma (lanes 3 and 6)cell line lysates: - 1-3. Anti-NETO2 (extracellular) Antibody (#AGC-046) (1:200).4-6. Anti-NETO2 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-163

SKU :

APC-163-CF_0.2 ml

Product Name :

Anti-KCNE1 (IsK) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P15383

Applications :

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC163

Homology :

Mouse, pig, dog - identical; human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RSKKLEHSHDPFN, corresponding to amino acid residues 68-80 of rat KCNE1

Product Page - Scientific background :

Potassium delayed rectifier channels are a sub-type of potassium channels found in various species of animals. They are responsible for the late-repolarization phase and the duration of action potential, especially in cardiac myocytes.There are two types of delayed rectifier channels (Ik). The Fast component is known as Ikr and is encoded by the gene KCNH2 in Xenopus. This channel's α-subunit is a 6-transmembrane domains voltage dependent potassium channel and its regulatory β-subunit, MiRP1, is required to reconstitute native Ikr1.KCNE1 and KCNQ1 are the genes encoding the two components of the Iks channel and this association was not fully understood for a long time. KCNQ1 (KV7.1) is a protein with six transmembrane domains and a typical pore loop containing a potassium signature sequence.The KNCE1 gene encodes the protein "minK". This gene is one of family of genes that encode small channel subunits (smaller than 130 amino-acids) that co-assemble with bigger subunits thus changing channel gating properties. These are currently the smallest known potassium channel subunits. Their small size and putative transmembrane domain prevent them from forming channels on their own. In addition, in an experiment where mammalian cells were transfected with minK, no K+ currents were observed. When transfecting cells with a combination of KNCE1 and KNCQ1 the currents observed were up to ten times larger than currents induced by the KNCQ1 subunit alone2.The loss of function of Iks is tightly linked with the long QT syndrome and bilateral deafness and gain of function was found to be linked with cardiac tachy-arrhythmias3,4.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Delayed rectifier potassium channel subunit IsK, Minimal potassium channel, MinK

Short Description :

A Rabbit Polyclonal Antibody to KCNE1

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KCNE1. Anti-KCNE1 (IsK) Antibody (#APC-163) can be used in western blot analysis. It has been designed to recognize KCNE1 from rat, mouse and human samples.

Negative Control :

BLP-PC163

Positive Control :

Synonyms :

Delayed rectifier potassium channel subunit IsK, Minimal potassium channel, MinK

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNE1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage