Anti-KV1.2 (KCNA2) (extracellular) Antibody | Alomone Labs APC-162 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV1.2 (KCNA2) (extracellular) Antibody

catalog :

APC-162

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Yang Y, Arsenault J, Bah A, Krzeminski M, Fekete A, Chao O, et al. Identification of a molecular locus for normalizing dysregulated GABA release from interneurons in the Fragile X brain. Mol Psychiatry. 2018;: pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes (lanes 1 and 4), mouse brain membranes (lanes 2 and 5) and rat brain membranes (lanes 3 and 6): - 1-3. Anti-KV1.2 (KCNA2) (extracellular) Antibody (#APC-162), (1:200).4-6. Anti-KV1.2 (KCNA2) (extracellular) Antibody, preincubated with Kv1.2/KCNA2 (extracellular) Blocking Peptide (#BLP-PC162).

image 2 :

Peptide (C)RDENEDMHGGGVT corresponding to amino acid residues 189-201 of rat KV1.2 (AccessionP63142). 1stextracellular loop.

image 3 :

Expression of KV1.2 in live intact rat PC12 cells - Cell surface detection ofKV1.2 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells withAnti-KV1.2 (KCNA2) (extracellular) Antibody (#APC-162) (1:50) followed by goat anti-rabbit-AlexaFluor-488 secondary antibody (green). B. Live view of the cells. C. Merge of A and B.

product information

CAT :

APC-162

SKU :

APC-162-CF_0.2 ml

Product Name :

Anti-KV1.2 (KCNA2) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P63142

Applications :

IC IF LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC162

Homology :

Mouse - identical; human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)RDENEDMHGGGVT, corresponding to amino acid residues 189-201 of rat KV1.2

Product Page - Scientific background :

KV1.2 is a mammalian voltage-dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.2 was first cloned from rat brain.1 Eight Shaker-related genes exist in mammals constituting the KV1 subfamily of the large KV channel family of genes.2A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.The structure of KV1.2 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2The channel is expressed in neurons and cardiac and smooth muscle tissue as well as in retina and pancreas.2 The crystal structure of KV1.2 was recently solved shading light on the structure of a mammalian voltage dependent channel.3 The functional channel is considered low voltage activated and shows very little inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.KV1.2 channels are sensitive to high doses of TEA (560 mM) and low doses of 4-AP (0.59 mM), the "classical" non-selective potassium channel blockers.Several venomous toxins from snakes, scorpions and honey bee are potent blockers (affecting the channels in the nanomolar range) of KV1.2 channels. Among these, the most potent and selective are α-Dendrotoxin (1-12 nM), Dendrotoxin-I (0.13 nM), Maurotoxin (0.1-0.8 nM), Hongotoxin-1 (0.17 nM), Margatoxin (0.16-0.65 nM), Tityustoxin Kα (0.21 nM) and MCD peptide (10-400 nM).4

Applications may also work in :

IC IF LCI WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily A member 2, RBK2

Short Description :

A Rabbit Polyclonal Antibody to KV1.2 (KCNA2) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KV1.2. Anti-KV1.2 (KCNA2) (extracellular) Antibody (#APC-162) can be used in western blot and live cell imaging applications. It has been designed to recognize KV1.2 from human, rat and mouse samples.

Negative Control :

BLP-PC162

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily A member 2, RBK2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNA2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage