Anti-KV1.1 (KCNA1) (extracellular) Antibody | Alomone Labs APC-161 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV1.1 (KCNA1) (extracellular) Antibody

catalog :

APC-161

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Liu W, Luque M, Li H, Schrott Fischer A, Glueckert R, Tylstedt S, et al. Spike Generators and Cell Signaling in the Human Auditory Nerve: An Ultrastructural, Super-Resolution, and Gene Hybridization Study. Front Cell Neurosci. 2021;15:642211 pubmed publisher
Tian Y, Korn P, Tripathi P, Komnig D, Wiemuth D, Nikouee A, et al. The mono-ADP-ribosyltransferase ARTD10 regulates the voltage-gated K+ channel Kv1.1 through protein kinase C delta. BMC Biol. 2020;18:143 pubmed publisher
Michelson N, Vazquez A, Eles J, Salatino J, Purcell E, Williams J, et al. Multi-scale, multi-modal analysis uncovers complex relationship at the brain tissue-implant neural interface: New Emphasis on the Biological Interface. J Neural Eng. 2017;: pubmed publisher
Vivekananda U, Novak P, Bello O, Korchev Y, Krishnakumar S, Volynski K, et al. Kv1.1 channelopathy abolishes presynaptic spike width modulation by subthreshold somatic depolarization. Proc Natl Acad Sci U S A. 2017;114:2395-2400 pubmed publisher
Fellerhoff Losch B, Korol S, Ganor Y, Gu S, Cooper I, Eilam R, et al. Normal human CD4(+) helper T cells express Kv1.1 voltage-gated K(+) channels, and selective Kv1.1 block in T cells induces by itself robust TNFÎ± production and secretion and activation of the NFÎºB non-canonical pathway. J Neural Transm (Vienna). 2016;123:137-57 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1,2. Anti-KV1.1 (KCNA1) (extracellular) Antibody (#APC-161), (1:200).3,4. Anti-KV1.1 (KCNA1) (extracellular) Antibody, preincubated with Kv1.1/KCNA1 (extracellular) Blocking Peptide (#BLP-PC161).

image 2 :

Western blot analysis of human MCF-7 breast adenocarcinoma cell lysate: - 1.Anti-KV1.1 (KCNA1) (extracellular) Antibody (#APC-161) (1:200).2. Anti-KV1.1 (KCNA1) (extracellular) Antibody preincubated with the negative control antigen.

image 3 :

Expression of KV1.1 in rat PC12 cells - Cell surface detection of KV1.1 in live intact rat PC12 pheochromocytoma cells. A. Extracellular staining of cells withAnti-KV1.1 (KCNA1) (extracellular) Antibody (#APC-161) (1:50) followed by goat anti-rabbit-AlexaFluor-594 secondary antibody (red). B. Live view of the cells. C. Merge of A and B.

product information

CAT :

APC-161

SKU :

APC-161-CF_0.2 ml

Product Name :

Anti-KV1.1 (KCNA1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P10499

Applications :

IC IF IFC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC161

Homology :

Mouse - identical; human - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KDDKDFTGTIHRID, corresponding to amino acid residues 193-206 of rat KV1.1

Product Page - Scientific background :

KV1.1 is a mammalian voltage-dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.1 was the first mammalian KV channel to be cloned from mouse brain.1 Eight Shaker-related genes exist in mammals constituting the KV1, subfamily of the large KV channel family of genes.2A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function. The structure of KV1.1 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2The channel is expressed in neurons and cardiac and skeletal muscle tissue as well as in the retina and pancreas.2 The functional channel is considered low voltage activated and shows very little inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle. Mutations in the coding of KV1.1 gene were discovered in Episodic Ataxia patients.3KV1.1 channels are sensitive to low doses of TEA (0.3 mM) and 4-AP (0.29 mM), the "classical" non-selective potassium channel blockers.Several venomous toxins from snakes, scorpions and sea anemones are potent blockers (affecting the channels in the nanomolar range) of KV1.1 channels. Among these, the most potent and selective are α-Dendrotoxin (0.4-4 nM) and δ-Dendrotoxin (0.03-1.8 nM), Dendrotoxin-K (0.03 nM), Agitoxin-2 (0.044 nM) and Hongotoxin-1 (0.031 nM).4

Applications may also work in :

IC IF IFC LCI WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily A member 1

Short Description :

A Rabbit Polyclonal Antibody to KV1.1 (KCNA1) Channel

Long Description :

Anti-KV1.1 (KCNA1) (extracellular) Antibody (#APC-161) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry and live cell imaging applications. It has been designed to recognize KV1.1 from human, rat and mouse samples.

Negative Control :

BLP-PC161

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily A member 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage