Anti-KCNQ5 Antibody | Alomone Labs APC-155 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNQ5 Antibody

catalog :

APC-155

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 7

Reference
Paz R, Tubert C, Stahl A, Díaz A, Etchenique R, Murer M, et al. Inhibition of striatal cholinergic interneuron activity by the Kv7 opener retigabine and the nonsteroidal anti-inflammatory drug diclofenac. Neuropharmacology. 2018;137:309-321 pubmed publisher
Ghezzi F, Monni L, Nistri A. Functional up-regulation of the M-current by retigabine contrasts hyperexcitability and excitotoxicity on rat hypoglossal motoneurons. J Physiol. 2018;596:2611-2629 pubmed publisher
Parrilla Carrero J, Buchta W, Goswamee P, Culver O, McKendrick G, Harlan B, et al. Restoration of Kv7 Channel-Mediated Inhibition Reduces Cued-Reinstatement of Cocaine Seeking. J Neurosci. 2018;38:4212-4229 pubmed publisher
Sakai A, Saitow F, Maruyama M, Miyake N, Miyake K, Shimada T, et al. MicroRNA cluster miR-17-92 regulates multiple functionally related voltage-gated potassium channels in chronic neuropathic pain. Nat Commun. 2017;8:16079 pubmed publisher
Aguayo Mazzucato C, van Haaren M, Mruk M, Lee T, Crawford C, Hollister Lock J, et al. β Cell Aging Markers Have Heterogeneous Distribution and Are Induced by Insulin Resistance. Cell Metab. 2017;25:898-910.e5 pubmed publisher
Wu Z, Li L, Xie F, Du J, Zuo Y, Frost J, et al. Activation of KCNQ Channels Suppresses Spontaneous Activity in Dorsal Root Ganglion Neurons and Reduces Chronic Pain after Spinal Cord Injury. J Neurotrauma. 2017;34:1260-1270 pubmed publisher
Chen X, Li W, Hiett S, Obukhov A. Novel Roles for Kv7 Channels in Shaping Histamine-Induced Contractions and Bradykinin-Dependent Relaxations in Pig Coronary Arteries. PLoS ONE. 2016;11:e0148569 pubmed publisher

image

image 1 :

Western blot analysis of rat hippocampus (lanes 1 and 4), mouse brain (lanes 2 and 5) and rat brain (lanes 3 and 6) lysates: - 1-3. Anti-KCNQ5 Antibody (#APC-155), (1:200).4-5. Anti-KCNQ5 Antibody, preincubated with KCNQ5 Blocking Peptide (#BLP-PC155).

image 2 :

Expression of KCNQ5 in rat hippocampus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-KCNQ5 Antibody (#APC-155), (1:100). A. KCNQ5 (green) is expressed in hilar cells of the hippocampal dentate gyrus. B. Double-staining of KCNQ5 (green) with a neuronal marker (“Milli-Mark” from Millipore, red) reveals partial localization with hilar interneurons (arrow points at an example).

product information

CAT :

APC-155

SKU :

APC-155-CF_0.2 ml

Product Name :

Anti-KCNQ5 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JK45

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC155

Homology :

Human, rat - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RGSQDFYPKWRESK, corresponding to amino acid residues 856-869 of mouse KCNQ5

Product Page - Scientific background :

KCNQ genes encode members of the KV7 K+ channels. Structurally, the KCNQ family belongs to the six transmembrane domain category of K+ channels. Five members belong to this family (KCNQ1-KCNQ5) of which KCNQ2 to KCNQ5 are expressed in the nervous system1-3. KCNQ5 is also expressed in visceral smooth muscle4 and skeletal muscle5.These channels form subunits of the low threshold voltage gated K+ channel. KCNQ2 and KCNQ3 mostly form heteromers to form the M current. KCNQ5 is known to contribute to this current1.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

KV7.5, Potassium voltage-gated channel subfamily KQT member 5, KQT-like 5, KvLQT5

Short Description :

A Rabbit Polyclonal Antibody to KCNQ5 (KV7.5) Channel

Long Description :

Anti-KCNQ5 Antibody (#APC-155) is a highly specific antibody directed against an epitope of the mouse KV7.5 channel. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KCNQ5 from rat, mouse, and human samples.

Negative Control :

BLP-PC155

Positive Control :

Synonyms :

KV7.5, Potassium voltage-gated channel subfamily KQT member 5, KQT-like 5, KvLQT5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNQ5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage