Anti-KCNMA1 (KCa1.1) (extracellular) Antibody | Alomone Labs APC-151 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNMA1 (KCa1.1) (extracellular) Antibody

catalog :

APC-151

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunohistochemistry - frozen section

more info or order :

Alomone Labs product webpage

citations: 12

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:500; loading ...; fig 4b	Chan C, Wu S, Bao B, Li H, Lu T. MST3 Involvement in Na+ and K+ Homeostasis with Increasing Dietary Potassium Intake. Int J Mol Sci. 2021;22: pubmed publisher
western blot; human; loading ...; fig s3e	Sun A, Yuan Q, Fukuda M, Yu W, Yan H, Lim G, et al. Potassium channel dysfunction in human neuronal models of Angelman syndrome. Science. 2019;366:1486-1492 pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; fig 5	Li Y, Hu H, Butterworth M, Tian J, Zhu M, O Neil R. Expression of a Diverse Array of Ca2+-Activated K+ Channels (SK1/3, IK1, BK) that Functionally Couple to the Mechanosensitive TRPV4 Channel in the Collecting Duct System of Kidney. PLoS ONE. 2016;11:e0155006 pubmed publisher
	Ohya S, Kajikuri J, Endo K, Kito H, Matsui M. KCa1.1 K+ Channel Inhibition Overcomes Resistance to Antiandrogens and Doxorubicin in a Human Prostate Cancer LNCaP Spheroid Model. Int J Mol Sci. 2021;22: pubmed publisher
	Ohya S, Kajikuri J, Endo K, Kito H, Elboray E, Suzuki T. Ca2+ -activated K+ channel KCa 1.1 as a therapeutic target to overcome chemoresistance in three-dimensional sarcoma spheroid models. Cancer Sci. 2021;112:3769-3783 pubmed publisher
	Sizemore G, McLaughlin S, Newman M, Brundage K, Ammer A, Martin K, et al. Opening large-conductance potassium channels selectively induced cell death of triple-negative breast cancer. BMC Cancer. 2020;20:595 pubmed publisher
	Balderas E, Torres N, Rosa Garrido M, Chaudhuri D, Toro L, Stefani E, et al. MitoBKCa channel is functionally associated with its regulatory β1 subunit in cardiac mitochondria. J Physiol. 2019;597:3817-3832 pubmed publisher
	Chubinskiy Nadezhdin V, Sudarikova A, Shilina M, Vasileva V, Grinchuk T, Lyublinskaya O, et al. Cell Cycle-Dependent Expression of Bk Channels in Human Mesenchymal Endometrial Stem Cells. Sci Rep. 2019;9:4595 pubmed publisher
	Lu M, Li J, Alvarez Lugo L, Li Y, Yu S, Li X, et al. Lipopolysaccharide stimulates BK channel activity in bladder umbrella cells. Am J Physiol Cell Physiol. 2018;314:C643-C653 pubmed publisher
	Comerma Steffensen S, Carvacho I, Hedegaard E, Simonsen U. Small and Intermediate Calcium-Activated Potassium Channel Openers Improve Rat Endothelial and Erectile Function. Front Pharmacol. 2017;8:660 pubmed publisher
	Baker D, Pryce G, Visintin C, Sisay S, Bondarenko A, Vanessa Ho W, et al. Big conductance calcium-activated potassium channel openers control spasticity without sedation. Br J Pharmacol. 2017;174:2662-2681 pubmed publisher
	Rosa P, Sforna L, Carlomagno S, Mangino G, Miscusi M, Pessia M, et al. Overexpression of Large-Conductance Calcium-Activated Potassium Channels in Human Glioblastoma Stem-Like Cells and Their Role in Cell Migration. J Cell Physiol. 2017;232:2478-2488 pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 1,2. Anti-KCNMA1 (KCa1.1) (extracellular) Antibody (#APC-151), (1:200).3,4. Anti-KCNMA1 (KCa1.1) (extracellular) Antibody, preincubated with KCNMA1/KCa1.1 (extracellular) Blocking Peptide (#BLP-PC151).

image 2 :

Western blot analysis of rat (lanes 1 and 3) and mouse brain membranes (lanes 2 and 4): - 12.Anti-KCNMA1 (KCa1.1) (extracellular)Antibody (#APC-151) (1:200).34. Anti-KCNMA1 (KCa1.1) (extracellular) Antibody preincubated with the negative control antigen.

image 3 :

Peptide (C)DSSNPIES(S)QNFYKD corresponding to amino acid residues 199-213 of rat KCNMA1(AccessionQ62976). 1stextracellular loop.

product information

CAT :

APC-151

SKU :

APC-151-CF_0.2 ml

Product Name :

Anti-KCNMA1 (KCa1.1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q62976

Applications :

IF IFC IHC LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC151

Homology :

Rat, mouse, human - 14/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DSSNPIES(S)QNFYKD, corresponding to amino acid residues 199-213 of rat KCNMA1

Product Page - Scientific background :

The KCa1.1 channel (also known as KCNMA1, BKCa, Maxi K+ or slo) is part of a structurally diverse group of K+ channels that are activated by an increase in intracellular Ca2+. KCa1.1 shows a large single channel conductance when recorded electrophysiologically and hence its name. It differs from the rest of the subfamily members in that it can be activated by both an increase in intracellular Ca2+ and by membrane depolarization. In addition, the KCa1.1 channel structurally differs from the other Ca2+-dependent K+ channels. While the latter group has a topology that resembles that of the voltage-dependent K+ channels, the KCa1.1 channel has an extracellular N-terminus domain as well as an additional transmembrane domain.KCa1.1 is expressed in virtually all cell types where it causes hyperpolarization and helps to connect intracellular Ca2+ signaling pathways and membrane excitability.Indeed, KCa1.1 channels play a crucial role in smooth muscle contractility, neuronal spike shaping and neurotransmitter release.

Applications may also work in :

IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1

Short Description :

A Rabbit Polyclonal Antibody to KCNMA1 (KCa1.1) Channel

Long Description :

Anti-KCNMA1 (KCa1.1) (extracellular) Antibody (#APC-151) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the channel in living cells. It has been designed to recognize KCNMA1 from human, rat, and mouse samples.

Negative Control :

BLP-PC151

Positive Control :

Synonyms :

Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNMA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage