Anti-KV1.5 (KCNA5) (extracellular) Antibody | Alomone Labs APC-150 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV1.5 (KCNA5) (extracellular) Antibody

catalog :

APC-150

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 9

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:1000; loading ...; fig 2c	Liu G, Papa A, Katchman A, Zakharov S, Roybal D, Hennessey J, et al. Mechanism of adrenergic CaV1.2 stimulation revealed by proximity proteomics. Nature. 2020;577:695-700 pubmed publisher
	Kilfoil P, Chapalamadugu K, Hu X, Zhang D, Raucci F, Tur J, et al. Metabolic regulation of Kv channels and cardiac repolarization by Kvβ2 subunits. J Mol Cell Cardiol. 2019;137:93-106 pubmed publisher
	Lamothe S, Hogan Cann A, Li W, Guo J, Yang T, Tschirhart J, et al. The N terminus and transmembrane segment S1 of Kv1.5 can coassemble with the rest of the channel independently of the S1-S2 linkage. J Biol Chem. 2018;293:15347-15358 pubmed publisher
	Cheng L, Al Owais M, COVARRUBIAS M, Koch W, Manning D, Peers C, et al. Coupling of Smoothened to inhibitory G proteins reduces voltage-gated K+ currents in cardiomyocytes and prolongs cardiac action potential duration. J Biol Chem. 2018;293:11022-11032 pubmed publisher
	Fu L, Lv Y, Zhong Y, He Q, Liu X, Du L. Tyrosine phosphorylation of Kv1.5 is upregulated in intrauterine growth retardation rats with exaggerated pulmonary hypertension. Braz J Med Biol Res. 2017;50:e6237 pubmed publisher
	Nystoriak M, Zhang D, Jagatheesan G, Bhatnagar A. Heteromeric complexes of aldo-keto reductase auxiliary KV? subunits (AKR6A) regulate sarcolemmal localization of KV1.5 in coronary arterial myocytes. Chem Biol Interact. 2017;276:210-217 pubmed publisher
	Nishijima Y, Cao S, Chabowski D, Korishettar A, Ge A, Zheng X, et al. Contribution of KV1.5 Channel to Hydrogen Peroxide-Induced Human Arteriolar Dilation and Its Modulation by Coronary Artery Disease. Circ Res. 2017;120:658-669 pubmed publisher
	JimÃ©nez PÃ©rez L, Cidad P, Ãlvarez Miguel I, Santos HipÃ³lito A, Torres Merino R, Alonso E, et al. Molecular Determinants of Kv1.3 Potassium Channels-induced Proliferation. J Biol Chem. 2016;291:3569-80 pubmed publisher
	Crump S, Hu Z, Kant R, Levy D, Goldstein S, Abbott G. Kcne4 deletion sex- and age-specifically impairs cardiac repolarization in mice. FASEB J. 2016;30:360-9 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KV1.5 (KCNA5) (extracellular) Antibody (#APC-150), (1:200).2. Anti-KV1.5 (KCNA5) (extracellular) Antibody, preincubated with Kv1.5/KCNA5 (extracellular) Blocking Peptide (#BLP-PC150).

image 2 :

Expression of KV1.5 channels in rat cerebellum - Immunohistochemical staining of rat cerebellum with Anti-KV1.5 (KCNA5) (extracellular) Antibody (#APC-150), (1:200). A. KV1.5 (green) appears in both the soma of Purkinje cells (horizontal arrows) and in Purkinje dendrites (vertical arrows). B. Neurons expressing gamma amino butyric acid (GABA) were labeled with mouse anti-parvalbumin antibody (red). C. Merge of the two images demonstrates partial colocalization (white arrows).

image 3 :

Cell surface detection of KV1.5 in live intact THP-1 (human acute monocytic leukemia cells) cell line: - ___Cells + Goat-anti-rabbit-Cy5.___Cells +Anti-KV1.5 (KCNA5) (extracellular) Antibody (#APC-150) (1:20) + goat-anti-rabbit-Cy5.

product information

CAT :

APC-150

SKU :

APC-150-CF_0.2 ml

Product Name :

Anti-KV1.5 (KCNA5) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P19024

Applications :

IC IF IFC IHC LCI WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC150

Homology :

Rat - 12/13 amino acid residues identical; human - 11/13 amino acid residues identical; mouse - 9/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)DERELLRHPPVP(K), corresponding to amino acid residues 268-279 of rat KV1.5

Product Page - Scientific background :

KV1.5 is a mammalian voltage dependent K+ channel, homologous to the Drosophila Shaker K+ channel. KV1.5 was first cloned from the rat brain.1 Eight Shaker related genes exist in mammals constituting the KV1 subfamily of the large KV channel family of genes.2A functional KV1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.The structure of KV1.5 channel is similar to all KV channels and includes six membrane spanning helices creating a voltage sensor domain and a pore domain.2The channel is expressed in cardiac and smooth muscle tissue (colon, aorta, stomach and pulmonary artery) as well as in neurons and kidney.2 A loss-of-function mutation in the gene encoding the channel was found in atrial fibrillation patients, stressing its role as a cardiac action potential regulator.3The functional channel is considered transient (A-type) channel and shows prominent inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.KV1.5 channels are sensitive to high doses of TEA (330 mM) and low doses of 4-AP (0.27 mM), the "classical" non-selective potassium channel blockers.2

Applications may also work in :

IC IF IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily A member 5

Short Description :

A Rabbit Polyclonal Antibody to KV1.5 (KCNA5) Channel

Long Description :

Anti-KV1.5 (KCNA5) (extracellular) Antibody (#APC-150) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, immunocytochemistry, and indirect flow cytometry. This antibody recognizes an extracellular epitope and thus is ideally suited to detect KV1.5 in living cells. It has been designed to recognize KV1.5 from human, mouse, and rat samples.

Negative Control :

BLP-PC150

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily A member 5

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNA5

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage