Anti-DPP10 (extracellular) Antibody | Alomone Labs APC-147 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-DPP10 (extracellular) Antibody

catalog :

APC-147

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate: - 1,2. Anti-DPP10 (extracellular) Antibody (#APC-147), (1:400).3,4. Anti-DPP10 (extracellular) Antibody, preincubated with DPP10 (extracellular) Blocking Peptide (#BLP-PC147).

image 2 :

Expression of DPP10 in mouse cerebellum and hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-DPP10 (extracellular) Antibody (#APC-147), (1:1000), followed by goat anti-rabbit-AlexaFluor-488. A. DPP10 immunoreactivity (green) in mouse cerebellum is detected in the Purkinje cell layer (P) in Purkinje cells (arrows) and diffusely in the molecular layer (MOL). B. DPP10 staining (green) in mouse hippocampal CA1 region is observed in the pyramidal layer (P, vertical arrow) and interneurons in the stratum oriens (SO, horizontal arrows). Cell nuclei are stained with DAPI (blue).

product information

CAT :

APC-147

SKU :

APC-147-CF_0.2 ml

Product Name :

Anti-DPP10 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6Q629

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC147

Homology :

Mouse - 14/15 amino acid residues identical; human - 12/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CSRKYEMTSDTWISK, corresponding to amino acid residues 356-370 of rat DPP10

Product Page - Scientific background :

Dipeptidyl peptidase 10 (DPP10, DPPY) is a member of the prolyl oligopeptidase family (shares a 30% sequence identity with DPP4) and belongs to the S9B serine protease subfamily. DPP10 is a homologous glycosylated, single-pass type II transmembrane protein that lacks the crucial serine residue of the catalytic triad. Unlike other KV4 subunits such as DPP6, DPP10 has a restricted expression pattern including the brain, adrenal gland and pancreas. The presence of DPP10 in endocrine cells suggests that the protein may also have an additional function related to the regulation of hormone secretion. DPP10 accelerates the activation and inactivation of KV4.3 channel gating with distinct biophysical properties.DPP10 associates with the α-subunits of the KV4 subfamily to form a ternary complex of approximately 750 kDa1. In addition, DPP10 forms complexes with other auxiliary subunits of the KV4 channels. In dorsal root ganglion DPP10 is co-localized with K+ channel-interacting proteins 1 and 2 (KChIPs) in the somatic surface and cytoplasm of KV4.3 nociceptors. Recently it has been hypothesized that KV4/KChIP/DPPL ternary complexes exist in subthreshold A-type K+ currents (Isas) expressing nociceptors and pain-modulating spinal interneurons2.DPP10 has been implicated in various pathologies. Aggregation of the protein is associated with neurodegenerative conditions such as Alzheimer's, diffuse Lewy body disease and fronto-temporal dementia3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Inactive dipeptidyl peptidase 10, Dipeptidyl peptidase X, DPP X, DPPY, DPL2, DPRP3

Short Description :

A Rabbit Polyclonal Antibody to Dipeptidyl Peptidase 10

Long Description :

Anti-DPP10 (extracellular) Antibody (#APC-147) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize DPP10 from rat, mouse, and human samples.

Negative Control :

BLP-PC147

Positive Control :

Synonyms :

Inactive dipeptidyl peptidase 10, Dipeptidyl peptidase X, DPP X, DPPY, DPL2, DPRP3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

DPP10

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:1000

Application Dilutions: Western blot wb :

1:200-1:400

more info or order :

Alomone Labs product webpage