Anti-DPP6 (extracellular) Antibody | Alomone Labs APC-146 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-DPP6 (extracellular) Antibody

catalog :

APC-146

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Shiotani H, Miyata M, Mizutani K, Wang S, Mizoguchi A, Mochizuki H, et al. Interaction of nectin-2α with the auxiliary protein of the voltage-gated A-type K+ channel Kv4.2 dipeptidyl aminopeptidase-like protein at the boundary between the adjacent somata of clustered cholinergic neurons in the medial habenula. Mol Cell Neurosci. 2019;94:32-40 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysate: - 1,2. Anti-DPP6 (extracellular) Antibody (#APC-146), (1:200).3,4. Anti-DPP6 (extracellular) Antibody, preincubated with DPP6 (extracellular) Blocking Peptide (#BLP-PC146).

image 2 :

Multiplex staining of DPP6 and KV4.2 in rat hippocampus - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using rabbit Anti-DPP6 (extracellular) Antibody (#APC-146), (1:400) and Guinea pig Anti-KV4.2 Antibody (#APC-023-GP), (1:1000). A. DPP6 (green) is expressed in the CA3 rat hippocampal region. B. KV4.2 (red) is expressed in the same region. C. Merge of the two images demonstrates partial colocalization in neurons of the CA3 region (arrows). Cell nuclei are stained with DAPI (blue).

image 3 :

Expression of DPP6 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-DPP6 (extracellular) Antibody(#APC-146) (1:400) followed by donkey anti-rabbit-Cy3 antibody (red). Staining in the hippocampal CA3 region appears in interneurons (arrows). DAPI is used to stain nuclei (blue).

product information

CAT :

APC-146

SKU :

APC-146-CF_0.2 ml

Product Name :

Anti-DPP6 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P42658

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC146

Homology :

Rat, mouse - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KKHEDESEAWLHR, corresponding to amino acid residues 414-426 of human DPP6

Product Page - Scientific background :

Dipeptidyl-peptidase 6 (DPP6) is a type II membrane glycoprotein1 which is a part of the dipeptydil aminopeptidase-like proteins (DPPLs) subfamily. It is an auxiliary glycoproteinic subunit of voltage-dependent K+ channel 4 (KV4.x), that has a short cytoplasmic domain, a long extracellular domain and a transmembrane domain. It regulates sub-threshold, A-type K+ currents, and is important for the enhancement and localization of KV4.2 channels2.DPPLs are a subgroup of the larger prolyl oligopeptidase family. A loss-of-function mutation at their enzymatic serine renders them incapable of cleaving peptides. Notable DPPLs include DPP8, DPP9, and DPPIV - the latter is known to regulate several key functions in varied systems, ranging from immune to CNS1,3.Alternative mRNA splicing of the DPP6 gene (part of the di-genic DPLP group that also consists DPP10) gives rise to several isoforms that differ in their N-terminal (DPP6a, DPP6K, DPP6S, DPP6L DPP6E)4.DPP6 proteins are widely expressed throughout both of the nervous systems - albeit much more predominantly in the CNS - and are known to play a major role in neurogenesis and neurodevelopment5.Deletion mutations and haplo-insufficiency of the DPP6 gene has been shown to be associated with autism spectrum disorder, Gilles de la Tourette syndrome, dominant microcephaly and mental retardation5,6. Endometriosis - a condition characterized by extra-uteral endometrial glands that affects one tenth of fertile women population - has been linked with DPP6 in genome wide associations studies (GWASs)7.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Dipeptidyl peptidase 6, Dipeptidyl aminopeptidase-like protein 6, DPP VI, DPPX, VF2

Short Description :

A Rabbit Polyclonal Antibody to Dipeptidyl Peptidase 6

Long Description :

Anti-DPP6 (extracellular) Antibody (#APC-146) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize DPP6 from rat, mouse, and human samples.

Negative Control :

BLP-PC146

Positive Control :

Synonyms :

Dipeptidyl peptidase 6, Dipeptidyl aminopeptidase-like protein 6, DPP VI, DPPX, VF2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

DPP6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage