Anti-KChIP3 (CSEN, DREAM) Antibody | Alomone Labs APC-143 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KChIP3 (CSEN, DREAM) Antibody

catalog :

APC-143

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human T-cell lymphoma Jurkat cell lysates (lanes 1 and 4), mouse heart lysates (lanes 2 and 5) and rat brain lysates (lanes 3 and 6): - 1-3. Anti-KChIP3 (CSEN, DREAM) Antibody (#APC-143), (1:200).4-6. Anti-KChIP3 (CSEN, DREAM) Antibody, preincubated with KChIP3/CSEN Blocking Peptide (#BLP-PC143).

image 2 :

Peptide (C)SKREGIKWQRPR corresponding to amino acid residues 18-31 of rat KChIP3 (AccessionQ9JM47). Cytoplasmic N-terminus.

image 3 :

Alomone Labs Conantokin G efficiently inhibits NMDA receptors expressed in Xenopus oocytes. - Representative NR1A/NR2B current elicited in Mg2+-free bath solution by continuous co-application of 1 M glutamate and 100 M glycine (black bar) and fully inhibited by 50 M Conantokin G (#STC-666) application (red bar). Membrane potential was held at -60 mV and current was recorded by two-electrode voltage-clamp.The figure was kindly provided by Prof. Shai Berlin Ruth and Bruce Rappaport Faculty of Medicine Technion Israel.

product information

CAT :

APC-143

SKU :

APC-143-CF_0.2 ml

Product Name :

Anti-KChIP3 (CSEN, DREAM) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9JM47

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC143

Homology :

Human, mouse - 11/12 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SKREGIKWQRPR, corresponding to amino acid residues 18-31 of rat KChIP3

Product Page - Scientific background :

Voltage-gated K+ (KV) channels form functional entities by the assembly of four α subunits and auxiliary subunits. Various auxiliary subunits are known to interact with KV channels thereby modulating various properties such as gating, activation and inactivation of the channels as well as influencing trafficking of the channels to the cell's plasma membrane1.KChIPs (KV channel interacting proteins) are cytoplasmic proteins which belong to the neural Ca2+ sensor (NCS) family of Ca2+ binding EF-hand proteins. To date, KChIP1-4 have been identified. All four KChIPs have a conserved C-terminal domain, which has four EF-hand-like Ca2+ binding motifs. The N-terminal region differs among the various KChIPs and attributes different properties regarding the regulation of KV channels1. KChIPs regulate different properties of KV channels such as their cell surface expression (mediated by proper trafficking of the various subunits), channel assembly and gating1-4. Specifically, KChIP2, 3 and 4 strongly regulate the activity of the KV4 channel family in cortical pyramidal neurons5. Furthermore, KChIP knockout mice display an increase in anxiety-like behavior compared to their wild type counterparts6.KChIP1, 3, and 4 are mostly expressed in the brain while KChIP2 is expressed in the heart as well as in the brain4-6.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Calsenilin, KCNIP3, KV channel-interacting protein 3, A-type potassium channel modulatory protein 3, DRE-antagonist modulator

Short Description :

A Rabbit Polyclonal Antibody to KChIP3 (CSEN, DREAM) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Calsenilin. Anti-KChIP3 (CSEN, DREAM) Antibody (#APC-143) can be used for western blot analysis. It has been designed to recognize Calsenilin from human mouse and rat samples.

Negative Control :

BLP-PC143

Positive Control :

Synonyms :

Calsenilin, KCNIP3, KV channel-interacting protein 3, A-type potassium channel modulatory protein 3, DRE-antagonist modulator

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNIP3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage