Anti-KChIP2 Antibody | Alomone Labs APC-142 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KChIP2 Antibody

catalog :

APC-142

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Zhang H, Zhang H, Wang C, Wang Y, Zou R, Shi C, et al. Auxiliary subunits control biophysical properties and response to compound NS5806 of the Kv4 potassium channel complex. FASEB J. 2020;34:807-821 pubmed publisher
Gao X, Gao S, Guan Y, Huang L, Huang J, Lin L, et al. Toll-like receptor 3 controls QT interval on the electrocardiogram by targeting the degradation of Kv4.2/4.3 channels in the endoplasmic reticulum. FASEB J. 2019;33:6197-6208 pubmed publisher
Choi B, Li W, Terentyev D, Kabakov A, Zhong M, Rees C, et al. Transient Outward K+ Current (Ito) Underlies the Right Ventricular Initiation of Polymorphic Ventricular Tachycardia in a Transgenic Rabbit Model of Long-QT Syndrome Type 1. Circ Arrhythm Electrophysiol. 2018;11:e005414 pubmed publisher

image

image 1 :

Western blot analysis of mouse brain lysate: - 1. Anti-KChIP2 Antibody (#APC-142), (1:200).2. Anti-KChIP2 Antibody, preincubated with KChIP2 Blocking Peptide (#BLP-PC142).

image 2 :

Expression of KChIP2 in mouse hippocampus - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Anti-KChIP2 Antibody (#APC-142), (1:400), followed by goat-anti-rabbit-Alexa-488 (green). KChIP2 staining in the hippocampal CA1 region appears in neuronal profiles (arrows) in the pyramidal layer (Pyr). DAPI is used to stain nuclei (blue).

image 3 :

Peptide (C)RGQGRKESLSDSRD corresponding to amino acid residues 2-15 of human KChIP2 (AccessionQ9NS61). Cytoplasmic N-terminus.

product information

CAT :

APC-142

SKU :

APC-142-CF_0.2 ml

Product Name :

Anti-KChIP2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9NS61

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC142

Homology :

Mouse - identical Rat - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Cytoplasmic, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RGQGRKESLSDSRD, corresponding to amino acid residues 2-15 of human KChIP2

Product Page - Scientific background :

KChIPs belong to the family of small cytosolic calcium-binding proteins and consist of four different KChIP isoforms ranging between 216 and 270 amino acids. They all have a reasonably conserved core region of 185 amino acids and a heterogeneous N-terminal. KChIPs have calcium-binding EF hands which appear to be important for modulation of KV channels but not for CaV channels1.The cardiac KChIP2 gene generates three protein isoforms of 220, 252, and 270 amino acids via alternative splicing of exons 2 and 3. It is well established that KChIP2 functionally increases peak KV4 current, slows channel inactivation and accelerates recovery from inactivation. Furthermore, KChIP2 has been reported to increase ICa,L and suggested to decrease KV1.5 cell surface expression and facilitate INa2 .All four KChIP mRNAs are found in the brain, however only KChIP2 is expressed in the heart3. KChIP2 is expressed heterogeneously across the canine and human left and right ventricular free wall with higher concentrations in epicardium compared to endocardium4.Mice deficient for KChIP2 exhibit normal cardiac structure and function but display a prolonged elevation in the ST segment on the electrocardiogram. The KChIP2−/− mice are highly susceptible to the induction of cardiac arrhythmias5.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

KCNIP2, KV channel-interacting protein 2, A-type potassium channel modulatory protein 2, Cardiac voltage-gated potassium channel modulatory subunit

Short Description :

A Rabbit Polyclonal Antibody to KChIP2 Subunit

Long Description :

Anti-KChIP2 Antibody (#APC-142) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KChIP2 from rat, mouse, and human samples.

Negative Control :

BLP-PC142

Positive Control :

Synonyms :

KCNIP2, KV channel-interacting protein 2, A-type potassium channel modulatory protein 2, Cardiac voltage-gated potassium channel modulatory subunit

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNIP2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage