Anti-KChIP1 (KCNIP1) Antibody | Alomone Labs APC-141 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KChIP1 (KCNIP1) Antibody

catalog :

APC-141

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Kuo Y, Cheng J, Hou W, Chang Y, Du P, Jian J, et al. K+ Channel Modulatory Subunits KChIP and DPP Participate in Kv4-Mediated Mechanical Pain Control. J Neurosci. 2017;37:4391-4404 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-KChIP1 (KCNIP1) Antibody (#APC-141), (1:200).2. Anti-KChIP1 (KCNIP1) Antibody, preincubated with KChIP1/KCNIP1 Blocking Peptide (#BLP-PC141).

image 2 :

Peptide (C)SLQTKQRRPSKD corresponding to amino acid residues 10-21 of rat KChIP1 (AccessionQ8R426). N-terminal part.

image 3 :

Western blot analysis of rat brain (lanes 1 and 4) mouse brain (lanes 2 and 5) and human U-87 MG glioblastoma cell (lanes 3 and 6) lysates: - 12. Guinea pigAnti-P2X4 Receptor Antibody (#AGP-085) (1:500).3. Guinea pigAnti-P2X4 Receptor Antibody (1:1000).4-6. Guinea pigAnti-P2X4 Receptor Antibody preincubated with the negative control antigen.

product information

CAT :

APC-141

SKU :

APC-141-CF_0.2 ml

Product Name :

Anti-KChIP1 (KCNIP1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q8R426

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC141

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

N-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)SLQTKQRRPSKD, corresponding to amino acid residues 10-21 of rat KChIP1

Product Page - Scientific background :

Voltage-gated K+ (KV) channels form functional entities by the assembly of four α subunits and auxiliary subunits. Various auxiliary subunits are known to interact with KV channels thereby modulating various properties such as gating, activation and inactivation of the channels as well as influencing trafficking of the channels to the cell's plasma membrane1.KChIPs (KV channel interacting proteins) are cytoplasmic proteins which belong to the neural Ca2+ sensor (NCS) family of Ca2+ binding EF-hand proteins. To date, KChIP1-4 have been identified. All four KChIPs have a conserved C-terminal domain, which has four EF-hand-like Ca2+ binding motifs. The N-terminal region differs among the various KChIPs and attributes different properties regarding the regulation of KV channels1. KChIPs regulate different properties of KV channels such as their cell surface expression (mediated by proper trafficking of the various subunits), channel assembly and gating1-4. Specifically, KChIP1 strongly regulates the activity of the KV4 channel family1. KChIP1 has also been found to assist GABA-mediated IPSCs (inhibitory postsynaptic currents) by increasing the release of presynaptic transmitter5. Furthermore, KChIP knockout mice display an increase in anxiety-like behavior compared to their wild type counterparts5.KChIP1, 3, and 4 are mostly expressed in the brain while KChIP2 is expressed in the heart as well as in the brain4-6.

Applications may also work in :

Supplier :

Alomone Labs

Target :

KV channel-interacting protein 1, VABP, Vesicle APC-binding protein, A-type potassium channel modulatory protein 1, Potassium channel-interacting protein 1

Short Description :

A Rabbit Polyclonal Antibody to KChIP1 Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KChIP1. Anti-KChIP1 (KCNIP1) Antibody (#APC-141) can be used for western blot analysis. It has been designed to recognize KChIP1 from human mouse and rat samples.

Negative Control :

BLP-PC141

Positive Control :

Synonyms :

KV channel-interacting protein 1, VABP, Vesicle APC-binding protein, A-type potassium channel modulatory protein 1, Potassium channel-interacting protein 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNIP1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage