Anti-Kv6.4 (KCNG4) Antibody | Alomone Labs APC-138 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Kv6.4 (KCNG4) Antibody

catalog :

APC-138

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysate (lanes 1 and 3) and rat brain lysates (lanes 2 and 4): - 1-2. Anti-Kv6.4 (KCNG4) Antibody (#APC-138), (1:200). 3-4. Anti-Kv6.4 (KCNG4) Antibody, preincubated with Kv6.4 (KCNG4) Blocking Peptide (BLP-PC138).

image 2 :

Expression of Kv6.4 in rat cerebral aqueduct. - Immunohistochemical staining of perfusion-fixed frozen rat brain sections Anti-Kv6.4 (KCNG4) Antibody (#APC-138), (1:600), followed by donkey anti-rabbit-biotin and Streptavidin-Cy3. A. Kv6.4 immunoreactivity (red) appears in the wall of the aqueduct (arrow). B. Pre-incubation of the antibody with Kv6.4 (KCNG4) Blocking Peptide (BLP-PC138), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

APC-138

SKU :

APC-138-CF_0.2 ml

Product Name :

Anti-Kv6.4 (KCNG4) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q80XM3

Applications :

IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC138

Homology :

Rat- 14 out of 15 amino acid residues identical Unlikely to recognize human Kv64

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RLRRLQNTNSASERE, corresponding to amino acid residues 472 - 486 of mouse KCNG4

Product Page - Scientific background :

Voltage-gated K+ (Kv) channels are potassium selective multimeric channels that change their conformation in response to changes in the membrane potential. Kv channels play a central role in the determination of cellular excitability due to their contribution to the shape, duration and frequency of action potentials. A wide range of physiological activities are regulated via KV actions including cell signaling, proliferation and migration1.Kv channels are divided into several subfamilies based in sequence homology. Members of the Kv5, Kv6, Kv8, and Kv9 subfamilies, are unable to form functional channels, even though they have the typical topology of a Kv α-subunit and have therefore been designated silent Kv (KvS) subunits. KV6.4 (also known as KCNG4) is part of the electrically silent KV6 subfamily that includes four members Kv6.1-Kv6.42. Members of the Kv6 subfamily selectively interact with members of the Kv2 subfamily, forming functional Kv2/KvS heterotetramers that possess unique biophysical and pharmacological properties 2. Heterotetramerization of KV6.4 with Kv2 results in the formation of functional channels. The stoichiometry of Kv2/Kv6 complex was recently found to be 2:2 stoichiometry with activation V1/2 of 5.7 ± 3.1 mV and inactivation V1/2 of -62.8 ± 1.6 mV3.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Potassium Voltage-Gated Channel Subfamily G Member 4, Voltage-gated potassium channel subunit Kv6.4

Short Description :

A Rabbit Polyclonal antibody to Kv6.4 (KCNG4)

Long Description :

Anti-Kv6.4 (KCNG4) Antibody (#APC-138) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kv6.4 from mouse and rat samples. The antibody will not recognize Kv6.4 from human samples.

Negative Control :

BLP-PC138

Positive Control :

Synonyms :

Potassium Voltage-Gated Channel Subfamily G Member 4, Voltage-gated potassium channel subunit Kv6.4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNG4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:600

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage