Anti-KCNG1 (KV6.1) (extracellular) Antibody | Alomone Labs APC-135 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNG1 (KV6.1) (extracellular) Antibody

catalog :

APC-135

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-KCNG1 (KV6.1) (extracellular) Antibody (#APC-135), (1:400).3,4. Anti-KCNG1 (KV6.1) (extracellular) Antibody, preincubated with KCNG1/Kv6.1 (extracellular) Blocking Peptide (#BLP-PC135).

image 2 :

Expression of KV6.1 potassium channel in rat cerebellum - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-KCNG1 (KV6.1) (extracellular) Antibody (#APC-135), (1:200). A. KV6.1 is detected in rat cerebellum (red). Staining is apparent in Purkinje neurons (arrow) and in the molecular layer (Mol). B. Cell nuclei in the same section are stained with DAPI (blue). C. Merge of the two images.

product information

CAT :

APC-135

SKU :

APC-135-CF_0.2 ml

Product Name :

Anti-KCNG1 (KV6.1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

A2BDX4

Applications :

IF IHC WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC135

Homology :

Rat - identical; human - 11/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

2nd extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)RRKPSTGNSYLDK, corresponding to amino acid residues 325-337 of mouse KV6.1

Product Page - Scientific background :

Voltage-gated K+ channels are transmembrane proteins consisting of four α-subunits arranged in a radially symmetric fashion around a central aqueous pore. Each α-subunit consists of six transmembrane segments (S1-S6) with cytoplasmic NH2- and COOH-termini.In mammals, a large family of genes encodes α-subunits: KV1 (homologous to Drosophila Shaker), KV2 (Shab), KV3 (Shaw), KV4 (Shal), KV5, KV6, and KV91. The sparsely populated KV channel subfamilies KV5 and KV6 each contain one member, KV5.1 and KV6.12. KV6 channels are electrically silent and no functional role has been suggested for these channels3. A recent study found that KV6.1 forms functional channels specifically with KV2.13. It was also found that KV2.1 and KV6.1 mRNAs colocalize to the piriform cortex, hippocampus, dendate gyrus and olfactory tubercle in the brain4 and to SA node, atria and ventricle in the heart5. This suggests that KV6.1 may be physiologically important in generating an increased number of KV channel phenotypes even though it does not itself form functional homomultimeric channels3.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily G member 1

Short Description :

A Rabbit Polyclonal Antibody to KCNG1 (KV6.1) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the mouse KV6.1 channel. Anti-KCNG1 (KV6.1) (extracellular) Antibody (#APC-135) can be used in western blot and immunohistochemistry applications. It is specially suited for live cell imaging. It has been designed to recognize KV6.1 from rat, mouse and human samples.

Negative Control :

BLP-PC135

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily G member 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNG1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage