Anti-KCNF1 (KV5.1) Antibody | Alomone Labs APC-134 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNF1 (KV5.1) Antibody

catalog :

APC-134

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of mouse brain lysate (lanes 1 and 4), human SHSY-5Y neuroblastoma cell line lysate (lanes 2 and 5) and rat brain membranes (lanes 3 and 6): - 1-3. Anti-KCNF1 (KV5.1) Antibody (#APC-134), (1:400).4-6. Anti-KCNF1 (KV5.1) Antibody, preincubated with KCNF1/Kv5.1 Blocking Peptide (#BLP-PC134).

product information

CAT :

APC-134

SKU :

APC-134-CF_0.2 ml

Product Name :

Anti-KCNF1 (KV5.1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q7TSH7

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC134

Homology :

Mouse, rat, human - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)K(S)VWKFLEKPESS, corresponding to amino acid residues 166-178 of mouse KV5.1

Product Page - Scientific background :

Voltage-gated K+ channels are involved in maintaining membrane potential and regulating cell volume. They are also important for determining the cellular response to the external stimuli of neurotransmitters and neuro-peptides1. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume.Voltage-gated K+ channels are transmembrane proteins consisting of four α-subunits arranged in a radially symmetric fashion around a central aqueous pore. Each α-subunit consists of six transmembrane segments (S1-S6) with cytoplasmic NH2- and COOH-termini2. In mammals, a large family of genes encodes α-subunits: KV1 (homologous to Drosophila Shaker), KV2 (Shab), KV3 (Shaw), KV4 (Shal), KV5, KV6, and KV83.New electrically silent α subunits have recently been cloned which constitute three new subfamilies: KV5.1, KV6.1, and KV8.1. These α subunits, although cannot generate K+ channel activity themselves, can modulate in a specific way the function of KV2.1 and KV2.2 subunits. They inhibit the KV2.1 and KV2.2 channels when expressed at high concentrations4.KV5.1 is expressed in the heart, skeletal muscle, brain, kidney, and pancreas. A recent study revealed the expression of KV5.1 in isolated urinary bladder smooth muscle (UBSM) myocytes5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily F member 1

Short Description :

A Rabbit Polyclonal Antibody to KCNF1 (KV5.1) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the mouse KV5.1 channel. Anti-KCNF1 (KV5.1) Antibody (#APC-134) can be used in western blot analysis. It has been designed to recognize KV5.1 from rat, mouse and human samples.

Negative Control :

BLP-PC134

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily F member 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNF1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage