Anti-KCNS3 (KV9.3) (extracellular) Antibody | Alomone Labs APC-133 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNS3 (KV9.3) (extracellular) Antibody

catalog :

APC-133

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat lung membranes (lanes 1 and 3) and mouse heart membranes (lanes 2 and 4): - 1,2. Anti-KCNS3 (KV9.3) (extracellular) Antibody (#APC-133), (1:200).3,4. Anti-KCNS3 (KV9.3) (extracellular) Antibody, preincubated with KCNS3/Kv9.3 (extracellular) Blocking Peptide (#BLP-PC133).

image 2 :

Expression of KV9.3 channel in rat cerebellum - Immunohistochemical staining of immersion-fixed, free floating rat brain frozen sections using Anti-KCNS3 (KV9.3) (extracellular) Antibody (#APC-133), (1:200). A. KV9.3 staining (red) is expressed in the molecular layer (M). B. DAPI counterstain (blue) displays the layout of cerebellar layers. C. Merge of A and B demonstrates restriction of KV9.3 expression to the molecular layer.

image 3 :

Expression of KV9.3 channel in rat cerebellum - Immunohistochemical staining of immersion-fixed free floating rat brain frozen sections usingAnti-KCNS3(KV9.3) (extracellular) Antibody(#APC-133) (1:200). A. KV9.3 staining (red) is expressed in the molecular layer (M). B. DAPI counterstain (blue) displays the layout of cerebellar layers. C. Merge of A and B demonstrates restriction of KV9.3 expression to the molecular layer.

product information

CAT :

APC-133

SKU :

APC-133-CF_0.2 ml

Product Name :

Anti-KCNS3 (KV9.3) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O88759

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC133

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EFQNEDGEVDDPVLE, corresponding to amino acid residues 209-223 of rat KCNS3

Product Page - Scientific background :

K+ channels are transmembrane proteins expressed in many excitable and non-excitable cells. Functional entities are formed by the tetrameric assembly of α subunits which could be done in a homomeric or heteromeric fashion. In addition, the association of β subunits is also required for the proper function of K+ channels. Various splice variants are also expressed, thereby complicating the picture1,2.K+ channels belonging to the KV9 subfamily resemble the delayed-rectifier class of K+ channel α subunits. These channels include six transmembrane domains, an ion selective pore, a leucine zipper and positively charged amino acids in S4 the voltage sensor domain. Interestingly, both KV9.1 and KV9.3 channels are electrically silent delayed rectifying K+ channels. However, they are responsible for modifying the activity of other K+ channels such as that of KV2.1, yielding currents different from those of KV2.1 on its own1.KV9.1 is mainly expressed in the brain, human lens epithelial cells, kidney, prostate and testis. That of KV9.3 is more generalized and ubiquitous1.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily S member 3

Short Description :

A Rabbit Polyclonal Antibody to KCNS3 (KV9.3) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an extracellular epitope of the rat KV9.3 channel. Anti-KCNS3 (KV9.3) (extracellular) Antibody (#APC-133) can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV9.3 from human mouse and rat samples.

Negative Control :

BLP-PC133

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily S member 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNS3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage