Anti-KCNE4 (MiRP3) Antibody | Alomone Labs APC-129 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNE4 (MiRP3) Antibody

catalog :

APC-129

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat pancreas membrane (lanes 1 and 3) and heart (lanes 2 and 4) lysates: - 1,2. Anti-KCNE4 (MiRP3) Antibody (#APC-129), (1:600).3,4. Anti-KCNE4 (MiRP3) Antibody, preincubated with KCNE4/MiRP3 Blocking Peptide (#BLP-PC129).

image 2 :

Peptide (C)GYMKSKRREKKSS corresponding to amino acid residues 56-68 of mouse KCNE4 (AccessionQ9WTW3). Intracellular C-terminus.

image 3 :

Peptide (C)RSGYRALPLGDFDR corresponding to amino acid residues 4-17 of mouse STOML1 (AccessionQ8CI66). Extracellular N-terminus.

product information

CAT :

APC-129

SKU :

APC-129-CF_0.2 ml

Product Name :

Anti-KCNE4 (MiRP3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9WTW3

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC129

Homology :

Rat, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)GYMKSKRREKKSS, corresponding to amino acid residues 56-68 of mouse KCNE4

Product Page - Scientific background :

The K+ voltage-gated channel subfamily E member (KCNE) family is a group of small, non-conducting, single transmembrane domain proteins that associate with pore-forming potassium channel subunits to form mixed complexes with unique characteristics1.Five different KCNE proteins have been described (KCNE1-5)2. The KCNE regulatory subunits are small proteins (14-20 kD) with a type-1 integral membrane topology. It is believed that both the cytoplasmic C-terminus tail and the transmembrane domain are necessary for the interaction with the α subunits1.MinK-related peptides (MiRPs) MiRP3, protein encoded by KCNE43. The importance of these proteins to normal physiology of the heart and nervous system is exposed by their association with clinical disorders such as congenital long QT syndrome, drug-induced cardiac arrhythmias, sensorineural deafness, and periodic paralysis4. In addition, MiRPs influence the normal physiology of endocrine and exocrine glands, intestinal secretion, and renal excretion. MiRP3 is found to co-localize with KV4.2 subunits that contribute to cardiac transient outward K+ currents5. Levels of the KCNE4 transcript in human cardiac ventricle are robust and increase in patients with congestive failure suggesting a regulatory function for MiRP3 in the heart6. In addition to regulating KV4.2, MiRP3 also regulates KCNQ1 and BK channels2,5.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Potassium channel subunit β MiRP3, MinK-related peptide 3, Potassium voltage-gated channel subfamily E member 4

Short Description :

A Rabbit Polyclonal Antibody to KCNE4 (MiRP3) Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse KCNE4. Anti-KCNE4 (MiRP3) Antibody (#APC-129) can be used in western blot analysis. It has been designed to recognize KCNE4 from rat, mouse and human samples.

Negative Control :

BLP-PC129

Positive Control :

Synonyms :

Potassium channel subunit β MiRP3, MinK-related peptide 3, Potassium voltage-gated channel subfamily E member 4

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNE4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:600

more info or order :

Alomone Labs product webpage