Anti-KVβ1 Antibody | Alomone Labs APC-127 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KVβ1 Antibody

catalog :

APC-127

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of human SH-SY5Y neuroblastoma cell lysate (lanes 1 and 4), mouse brain lysate (lanes 2 and 5) and rat brain lysate (lanes 3 and 6): - 1-3. Anti-KVβ1 Antibody (#APC-127), (1:200).4-6. Anti-KVβ1 Antibody, preincubated with Kvβ1 Blocking Peptide (#BLP-PC127).

image 2 :

Peptide (C)TPQHHISLKESTAK corresponding to amino acid residues 54-67 of rat KCNAB1(AccessionP63144). N-terminus.

product information

CAT :

APC-127

SKU :

APC-127-CF_0.2 ml

Product Name :

Anti-KVβ1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P63144

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC127

Homology :

Mouse, human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

N-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)TPQHHISLKESTAK, corresponding to amino acid residues 54-67 of rat KCNAB1

Product Page - Scientific background :

Voltage-gated K+ channels represent a structurally and functionally diverse group of membrane proteins. These channels establish the resting membrane potential and modulate the frequency and duration of action potentials in nerve and muscle1. The duration of the open states of K+ channels is determined by the rates of deactivation and inactivation. KV channels are hetero-oligomeric complexes consisting of two different types of subunits: membrane-bound, pore-forming α subunits and the smaller β subunits. The β subunits appear to be peripheral proteins tightly associated with the cytoplasmic side of the α subunits2. The inactivation gate can be formed by an N-terminal segment of an auxiliary β-subunit, as in the complex of KVβ1 and KVα family channels3. There are three mammalian KVβ genes: KVβ1, KVβ2 and KVβ3. Additional variability in the KVβ1 family results from alternative splicing in the amino terminal region, thus yielding the KVβ1.1, KVβ1.2, and KVβ1.3 subunits4.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Voltage-gated potassium channel subunit beta-1, KV beta 1, KVb1, KCNAB1

Short Description :

A Rabbit Polyclonal Antibody to KVβ1 Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KVβ1. Anti-KVβ1 Antibody (#APC-127) can be used in western blot analysis. This antibody will recognize the splice isoform of human KVβ1 (also known as KVβ1.1). It won’t recognize splice variants KVβ1.2 and KVβ1.3. For more information, see human KVβ1 entry Q14722 and Q14722-2. It has been designed to recognize KVβ1 from rat, mouse and human samples.

Negative Control :

BLP-PC127

Positive Control :

Synonyms :

Voltage-gated potassium channel subunit beta-1, KV beta 1, KVb1, KCNAB1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNAB1

AB Specifictiy :

This antibody will recognize the "canonical" splice isoform of human KVβ1 (also known as KVβ1.3). It will not recognize splice variants KVβ1.1 and KVβ1.2. For more information see human KVβ1 entry Q14722.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage