Anti-KCNT2 (Slick) Antibody | Alomone Labs APC-126 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNT2 (Slick) Antibody

catalog :

APC-126

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Joseph L, Reyes M, Homan E, Gowen B, Avula U, Goulbourne C, et al. The mitochondrial calcium uniporter promotes arrhythmias caused by high-fat diet. Sci Rep. 2021;11:17808 pubmed publisher
Benzaquen J, Dit Hreich S, Heeke S, Juhel T, Lalvee S, Bauwens S, et al. P2RX7B is a new theranostic marker for lung adenocarcinoma patients. Theranostics. 2020;10:10849-10860 pubmed publisher
Ferreira J, Butler A, Stewart R, Gonzalez Cota A, Lybaert P, Amazu C, et al. Oxytocin can regulate myometrial smooth muscle excitability by inhibiting the Na+ -activated K+ channel, Slo2.1. J Physiol. 2019;597:137-149 pubmed publisher
Li P, Stewart R, Butler A, Gonzalez Cota A, Harmon S, Salkoff L. GABA-B Controls Persistent Na+ Current and Coupled Na+-Activated K+ Current. Eneuro. 2017;4: pubmed publisher

image

image 1 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4) brain membranes: - 1,2. Anti-KCNT2 (Slick) Antibody (#APC-126), (1:200).3,4. Anti-KCNT2 (Slick) Antibody, preincubated with KCNT2/Slick Blocking Peptide (#BLP-PC126).

image 2 :

Peptide (C)KDVKDPGHHRSIHR corresponding to amino acid residues 991-1004 of rat Slick(AccessionQ6UVM4). Intracellular C-terminus.

image 3 :

Western blot analysis of rat (lanes 1 and 3) and mouse (lanes 2 and 4)brain membranes: - 12.Anti-Synapsin II (SYN2) Antibody(#ANR-015) (1:200).34. Anti-Synapsin II (SYN2) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-126

SKU :

APC-126-CF_0.2 ml

Product Name :

Anti-KCNT2 (Slick) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6UVM4

Applications :

IC IF WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC126

Homology :

Mouse - 13/14 amino acid residues identical; human - 10/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KDVKDPGHHRSIHR, corresponding to amino acid residues 991-1004 of rat Slick

Product Page - Scientific background :

KCa4.1 (Na+-activated K+ channel, Slack, Slo2.2, KCNT1) was originally cloned and named so due to its high similarity to the Slo genes1. Shortly after its discovery, KCa4.2 (Slick, Slo2.1, KCNT2), its sister channel, was also cloned2. Although KCa4.2 (like KCa4.1) is functionally a Na+-activated K+ channel (KNa), it is termed KCa by the IUPHAR nomenclature, due to its sequence homology to other KCa channels. Both channels are activated by high intracellular concentrations of Na+.Like Slack, Slick contains six transmembrane spanning domains, a P-region between transmembrane regions 5 and 6 and intracellular N- and C-termini. However, the N-terminal domain of Slick is significantly shorter than that of Slack. In addition, contrary to Slack, Slick is regulated by ATP as it has an ATP binding site in its C-terminal domain2. ATP binding reduces the activity of the channel and mutations of this site abolish the inhibitory effect2. Both channels are regulated by intracellular Cl- ions, but Slick displays higher sensitivity3. Also, the overall electrical characteristics of Slick channels are different from those of Slack; Slick is rapidly activated in response to depolarization, and also has a basal level of activity in the absence of Na+. Both channels are highly expressed in the brain with overlapping expression. Slick is found in the midbrain, brainstem, and hippocampus and throughout the neocortex. This KNa channel is also detected in the auditory neurons in the brainstem. Detection of Slick was also found in the heart, although at much lower levels2.Many different functions have been attributed to KNa channels including action potential repolarization, slow after-hyperpolarization, burst firing and adaptation after repetitive firing3. These channels also contribute to the response of neurons to hypoxia.

Applications may also work in :

IC IF WB

Supplier :

Alomone Labs

Target :

KCa4.2, Slo2.1 Na+ and Cl--activated K+ channel, Potassium channel subfamily T member 2

Short Description :

A Rabbit Polyclonal Antibody to KCNT2 (Slick)

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal domain of rat KCNT2. Anti-KCNT2 (Slick) Antibody (#APC-126) can be used in western blot and immunocytochemistry applications. It has been designed to recognize KCNT2 from rat, human, and mouse samples.

Negative Control :

BLP-PC126

Positive Control :

Synonyms :

KCa4.2, Slo2.1 Na+ and Cl--activated K+ channel, Potassium channel subfamily T member 2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNT2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage