Anti-Kir7.1 (extracellular) Antibody | Alomone Labs APC-125 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Kir7.1 (extracellular) Antibody

catalog :

APC-125

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Papanikolaou M, Lewis A, Butt A. Glial and neuronal expression of the Inward Rectifying Potassium Channel Kir7.1 in the adult mouse brain. J Anat. 2019;235:984-996 pubmed publisher
Ambite I, Butler D, Stork C, Gr nberg Hern ndez J, K ves B, Zdziarski J, et al. Fimbriae reprogram host gene expression - Divergent effects of P and type 1 fimbriae. PLoS Pathog. 2019;15:e1007671 pubmed publisher
Shahi P, Liu X, Aul B, Moyer A, Pattnaik A, Denton J, et al. Abnormal Electroretinogram after Kir7.1 Channel Suppression Suggests Role in Retinal Electrophysiology. Sci Rep. 2017;7:10651 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membrane (lanes 1 and 4), mouse brain (lanes 2 and 5) and mouse kidney lysates (lanes 3 and 6): - 1-3. Anti-Kir7.1 (extracellular) Antibody (#APC-125), (1:200).4-6. Anti-Kir7.1 (extracellular) Antibody, preincubated with Kir7.1 (extracellular) Blocking Peptide (#BLP-PC125).

image 2 :

Expression of Kir7.1 in rat kidney - Immunohistochemical staining of paraffin embedded section of rat kidney using Anti-Kir7.1 (extracellular) Antibody (#APC-125), (1:100). Staining is present in both distal (DT) and proximal (PT) tubules and in the collecting ducts (CD) in the renal cortex. Note that staining is absent both in glomeruli (Gl) and blood vessels (arrow). Hematoxilin is used as the counterstain.

image 3 :

Cell surface detection of Kir7.1 in live intact Jurkat (human T cell leukemia) cell line: - ___Cells + Goat-anti-Rabbit-FITC.___Cells +Anti-Kir7.1 (extracellular)Antibody (#APC-125) (1:20) + Goat-anti-Rabbit-FITC.

product information

CAT :

APC-125

SKU :

APC-125-CF_0.2 ml

Product Name :

Anti-Kir7.1 (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O70617

Applications :

IFC IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC125

Homology :

Mouse - identical; human - 13/15 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EMNGDLEIDHDVPPE, corresponding to amino acid residues 80-94 of rat KCNJ13

Product Page - Scientific background :

Kir7.1 (KCNJ13) is a member of the inward rectifying K+ channel family. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's protein topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels, the functional unit for the Kir channels is composed of four subunits that can assemble as either homo- or heteromers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis1.Kir7.1, an inwardly rectifying K+ channel with unusual permeation properties is localized in epithelial cells of the thyroid, small intestine, kidney tubules, choroid plexus and in retinal pigment epithelium (RPE), where it forms a major component of the apical membrane K+ conductance2.A mutation in the gene encoding the channel was found to cause snowflake vitreoretinal degeneration (SVD) which is a developmental and progressive hereditary eye disorder that affects multiple tissues within the eye3.

Applications may also work in :

IFC IHC WB

Supplier :

Alomone Labs

Target :

Inward rectifier potassium channel 13, KCNJ13

Short Description :

A Rabbit Polyclonal Antibody to Kir7.1 Channel

Long Description :

Anti-Kir7.1 (extracellular) Antibody (#APC-125) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry and indirect flow cytometry applications. The antibody is directed against an extracellular epitope and is thus ideal for detecting Kir7.1 in living cells. It has been designed to recognize Kir7.1 from rat, mouse and human samples.

Negative Control :

BLP-PC125

Positive Control :

Synonyms :

Inward rectifier potassium channel 13, KCNJ13

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ13

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage