antibody

Anti-KCNT1 (Slack) Antibody

APC-124

polyclonal

domestic rabbit

nonconjugated

NA

mouse, rat

western blot, immunohistochemistry

APC-124-CF_0.2 ml

Anti-KCNT1 (Slack) Antibody

Antibodies

Antibodies

Polyclonal

Q9Z258

IHC WB

Rat Mouse

Rabbit

BLP-PC124

Mouse - 12/13 amino acid residues identical

PBS pH7.4

Rabbit IgG

Confirmed by amino acid analysis and mass spectrometry

0.2 ml double distilled water (DDW).

1 mg/ml

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

No Preservative

Intracellular, C-terminus

Unconjugated

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

Rat

(C)KQEEKQNRRGLAG, corresponding to amino acid residues 619-631 of rat Slack

KCa4.1 (Na+-activated K+ channel, Slack, Slo2.2, KCNT1) is a member of a subgroup within the Ca2+ activated K+ channel family. The Slack (Sequence like a Ca2+-activated K+ channel) and Slick (Sequence like an intermediate conductance K+ channel, KCNT2) genes, which encode Na+-activated K+ (KNa) channels, are expressed at high levels in neurons in several areas of the nervous system1.Although KCa4.1 is functionally a Na+-activated K+ channel, it is termed KCa by the IUPHAR nomenclature, due to its sequence homology to other KCa channels.The family's protein topology consists of six transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels, the functional unit for KCa4.1 channels is composed of four subunits that can assemble as either homo- or heteromers with KCNT2 (Slick). KCa4.1 and Slick subunits coassemble to form heteromeric channels that differ from the homomers in their unitary conductance, kinetic behavior, subcellular localization, and response to activation of protein kinase C. Heteromer formation requires the N-terminal domain of Slack-B, one of the alternative splice variants of the KCa4.1 channel. This cytoplasmic N-terminal domain of Slack-B also facilitates the localization of heteromeric KNa channels to the plasma membrane2.K+ channels sensitive to intracellular Na+, termed KNa channels, shape neuronal excitability. KNa channels contribute to the adaptation of firing rates and to slow after-hyperpolarizations that follow repetitive firing. In certain neurons, they also appear to be activated by Na+ influx accompanying single action potentials. Their molecular properties also suggest that these channels contribute to the response of neurons to hypoxia1. KCa4.1 channels were shown to be expressed also in dorsal root ganglion (DRG) neurons, where these channels were found to contain a NAD+ binding sequence and to be modulated by this endogenous compound3.

IHC WB

Alomone Labs

KCa4.1, KNa1.1, Slo2.2 Na+-activated K+ channel, Potassium channel subfamily T member 1

A Rabbit Polyclonal Antibody to KCNT1 (Slack)

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal domain of rat KCNT1. Anti-KCNT1 (Slack) Antibody (#APC-124) can be used in western blot applications, and was designed to recognize KCNT1 from rat and mouse samples.

BLP-PC124

NA

KCa4.1, KNa1.1, Slo2.2 Na+-activated K+ channel, Potassium channel subfamily T member 1

1-2 Business Days

Israel/IL

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

KCNT1

Unlikely to recognize human samples.

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Contact Alomone Labs for technical support and product customization

H- Human, M- Mouse, R- Rat

No

Affinity purified on immobilized antigen.

41116161

NA

Western blot analysis

1 µg peptide per 1 µg antibody

1:300

1:200