Anti-Kir5.1 Antibody | Alomone Labs APC-123 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Kir5.1 Antibody

catalog :

APC-123

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 7

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; mouse; 1:1000; loading ...; fig 1c	Poli G, Hasan S, Belia S, Cenciarini M, Tucker S, Imbrici P, et al. Kcnj16 (Kir5.1) Gene Ablation Causes Subfertility and Increases the Prevalence of Morphologically Abnormal Spermatozoa. Int J Mol Sci. 2021;22: pubmed publisher
	Lu J, Azad M, Moreau J, Zhu Y, Jiang X, Tonta M, et al. Inwardly rectifying potassium channels mediate polymyxin-induced nephrotoxicity. Cell Mol Life Sci. 2022;79:296 pubmed publisher
	Papanikolaou M, Lewis A, Butt A. Glial and neuronal expression of the Inward Rectifying Potassium Channel Kir7.1 in the adult mouse brain. J Anat. 2019;235:984-996 pubmed publisher
	Schirmer L, Mobius W, Zhao C, Cruz Herranz A, Ben Haim L, Cordano C, et al. Oligodendrocyte-encoded Kir4.1 function is required for axonal integrity. elife. 2018;7: pubmed publisher
	Wang M, Su X, Wu P, Gao Z, Wang W, Staub O, et al. Kir5.1 regulates Nedd4-2-mediated ubiquitination of Kir4.1 in distal nephron. Am J Physiol Renal Physiol. 2018;315:F986-F996 pubmed publisher
	Puissant M, Mouradian G, Liu P, Hodges M. Identifying Candidate Genes that Underlie Cellular pH Sensitivity in Serotonin Neurons Using Transcriptomics: A Potential Role for Kir5.1 Channels. Front Cell Neurosci. 2017;11:34 pubmed publisher
	Brasko C, Hawkins V, De La Rocha I, Butt A. Expression of Kir4.1 and Kir5.1 inwardly rectifying potassium channels in oligodendrocytes, the myelinating cells of the CNS. Brain Struct Funct. 2017;222:41-59 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 3) and liver (lanes 2 and 4) membranes: - 1,2. Anti-Kir5.1 Antibody (#APC-123), (1:200).3,4. Anti-Kir5.1 Antibody, preincubated with Kir5.1 Blocking Peptide (#BLP-PC123).

image 2 :

Expression of Kir5.1 in rat kidney - Immunohistochemical staining of paraffin embedded section of rat kidney using Anti-Kir5.1 Antibody (#APC-123) (1:100). Kir5.1 (brown staining) is expressed in both proximal tubules (PT) and distal tubules (DT) in the renal cortex. Note that collecting ducts (CD) are less stained while glomeruli (G) are negative. Hematoxilin is used as the counterstain.

image 3 :

Western blot analysis of rat kidney (lanes 1 and 3) and liver (lanes 2 and 4) membranes: - 12.Anti-Kir5.1Antibody (#APC-123) (1:200).34.Anti-Kir5.1 Antibody preincubated with the control peptide antigen.

product information

CAT :

APC-123

SKU :

APC-123-CF_0.2 ml

Product Name :

Anti-Kir5.1 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P52191

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC123

Homology :

Mouse - identical; human 14/15 amino acid residues-identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

HDVLEVKRKYYKVNC, corresponding to amino acid residues 311-325 of rat KCNJ16

Product Page - Scientific background :

Kir5.1 is a member of the family of inward rectifying K+ channels. The family includes 15 members that are structurally and functionally different from the voltage-dependent K+ channels.The family's protein topology consists of two transmembrane domains that flank a single and highly conserved pore region with intracellular N- and C-termini. As is the case for the voltage-dependent K+ channels the functional unit for the Kir channels is composed of four subunits that can assemble as either homo- or heteromers.Kir channels are characterized by a K+ efflux that is limited by depolarizing membrane potentials thus making them essential for controlling resting membrane potential and K+ homeostasis.1Kir5.1 can co-assemble with other Kir channels such as Kir2.1, Kir4.1 and Kir4.2. In fact, heteromeric Kir4.1/Kir5.1 form the basolateral small-conductance K+ channel in distal nephron segments that is responsible for generating the basolateral membrane potential that determines the magnitude and direction of K+ diffusion from cell to peritubular fluid in the nephron.2The Kir5.1 homomeric channel was largely thought to be non-functional, although recent evidence demonstrates that Kir5.1 homomers can form functional channels when co-expressed with the anchor protein PSD-95 in the brain where it is abundantly expressed.3

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Inward rectifier potassium channel 16, KCNJ16, BIR9

Short Description :

A Rabbit Polyclonal Antibody to Kir5.1 Channel

Long Description :

Anti-Kir5.1 Antibody (#APC-123) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kir5.1 from rat, mouse, and human samples.

Negative Control :

BLP-PC123

Positive Control :

Synonyms :

Inward rectifier potassium channel 16, KCNJ16, BIR9

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ16

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage