Anti-KCNK18 (TRESK) (extracellular) Antibody | Alomone Labs APC-122 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNK18 (TRESK) (extracellular) Antibody

catalog :

APC-122

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Liu J, Jing H, Xi K, Zhang Z, Jin Z, Cai S, et al. Contribution of TRESK two-pore domain potassium channel to bone cancer-induced spontaneous pain and evoked cutaneous pain in rats. Mol Pain. 2021;17:17448069211023230 pubmed publisher
Grabauskas G, Wu X, Zhou S, Li J, Gao J, Owyang C. High-fat diet-induced vagal afferent dysfunction via upregulation of 2-pore domain potassium TRESK channel. JCI Insight. 2019;4: pubmed publisher
Fischer R, Roux A, Wareham L, Sappington R. Pressure-dependent modulation of inward-rectifying K+ channels: implications for cation homeostasis and K+ dynamics in glaucoma. Am J Physiol Cell Physiol. 2019;317:C375-C389 pubmed publisher
Yang Y, Li S, Jin Z, Jing H, Zhao H, Liu B, et al. Decreased abundance of TRESK two-pore domain potassium channels in sensory neurons underlies the pain associated with bone metastasis. Sci Signal. 2018;11: pubmed publisher

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 3) and dorsal root ganglion (lanes 2 and 4) membranes: - 1,2. Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122), (1:200).3,4. Anti-KCNK18 (TRESK) (extracellular) Antibody, preincubated with KCNK18/TRESK (extracellular) Blocking Peptide (#BLP-PC122).

image 2 :

Expression of KCNK18 in rat dorsal root ganglia - Immunohistochemical staining of rat dorsal root ganglia (DRG) frozen sections using Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122), (1:100) followed by Alexa 555-labeled secondary antibody (red staining). Both big/medium sized neurons and small neurons are stained. Note that glial cells and axonal fibers are not stained. Hoechst 33342 is used as the counterstain (blue staining).

image 3 :

Peptide (C)EAEENPELKKFLDD corresponding to amino acid residues 62-75 of mouse KCNK18 (AccessionQ6VV64).1stextracellular loop.

product information

CAT :

APC-122

SKU :

APC-122-CF_0.2 ml

Product Name :

Anti-KCNK18 (TRESK) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q6VV64

Applications :

IC IF IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC122

Homology :

Rat - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)EAEENPELKKFLDD, corresponding to amino acid residues 62-75 of mouse KCNK18

Product Page - Scientific background :

KCNK18 (also named TWIK-related spinal cord K+ channel, TRESK or K2P18.1) is a member of the 2-pore (2P) domain K+ channel family that currently includes 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and control cell excitation.1The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. It has been proposed that the functional channel unit is a dimer.Different K2P family members are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, signaling pathways (PKC and PKA), arachidonic acid, etc.KCNK18 is the only K2P channel, known so far, whose current is activated following Gαq-receptor coupled activation. The enhancement of KCNK18 current involves activation of calcineurin (calcium-calmodulin-dependent phosphatase 2B) following the rise in intracellular calcium that occurs subsequent to Gαq activation.2 In addition, KCNK18 is potently activated by clinical concentrations of volatile anesthetics.3KCNK18 expression in humans is largely restricted to the spinal cord although in rodents it has a broader expression pattern that includes brain, testis and spleen.KCNK18 represents the most important background K+ channel in dorsal root ganglion neurons and hence it has been postulated that it has an important role in acute and chronic pain as well as general anesthesia.

Applications may also work in :

IC IF IHC LCI WB

Supplier :

Alomone Labs

Target :

Potassium channel subfamily K member 18, TWIK-related spinal cord K+ channel, K2P18.1

Short Description :

A Rabbit Polyclonal Antibody to KCNK18 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of mouse KCNK18. Anti-KCNK18 (TRESK) (extracellular) Antibody (#APC-122) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize TRESK channel from mouse and rat samples. The antibody will unlikely recognize TRESK from human samples.

Negative Control :

BLP-PC122

Positive Control :

Synonyms :

Potassium channel subfamily K member 18, TWIK-related spinal cord K+ channel, K2P18.1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNK18

AB Specifictiy :

Unlikely to recognize human samples.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage