Anti-KCNK13 (THIK-1) (extracellular) Antibody | Alomone Labs APC-121 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNK13 (THIK-1) (extracellular) Antibody

catalog :

APC-121

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat kidney membranes: - 1. Anti-KCNK13 (THIK-1) (extracellular) Antibody (#APC-121), (1:200).2. Anti-KCNK13 (THIK-1) (extracellular) Antibody, preincubated with KCNK13/THIK-1 (extracellular) Blocking Peptide (#BLP-PC121).

image 2 :

Expression of KCNK13 in rat kidney - Immunohistochemical staining in paraffin-embedded sections of rat kidney using Anti-KCNK13 (THIK-1) (extracellular) Antibody (#APC-121), (1:50). Staining (brown color) is specific for renal tubules while both glomeruli (G) and arterial walls are negative (red arrows). Following Tris-EDTA treatment for antigen retrieval, slides were incubated overnight at 4°C. Hematoxylin is used as the counterstain.

image 3 :

Peptide (C)EERLANFSRGHNLSRE corresponding to amino acid residues 54-69 of rat KCNK13(AccessionQ9ERS0).1stextracellularloop.

product information

CAT :

APC-121

SKU :

APC-121-CF_0.2 ml

Product Name :

Anti-KCNK13 (THIK-1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9ERS0

Applications :

IFC IHC LCI WB

Reactivity :

Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC121

Homology :

Mouse - identical; human - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

1st extracellular loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)EERLANFSRGHNLSRE, corresponding to amino acid residues 54-69 of rat KCNK13

Product Page - Scientific background :

KCNK13 (also named Tandem pore domain halotane inhibited K+ channel, THIK -1 or K2P13.1) is a member of the 2-pore (2P) domain K+ channels family that currently includes 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation.1The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini. It has been proposed that the functional channel unit is a dimer.K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. but are insensitive to classical K+ channel blockers.The KCNK13 channel is activated by arachidonic acid and inhibited by the volatile anesthetic halothane. The channel is relatively widely expressed in peripheral tissues such as kidney, heart, lung and liver but it is also expressed in brain.The physiological function of the KCNK13 channel is not clear but an involvement in the physiological response to reduced oxygen levels (hypoxia) has been proposed.2

Applications may also work in :

IFC IHC LCI WB

Supplier :

Alomone Labs

Target :

Potassium channel subfamily K member 13, Tandem pore domain halothane-inhibited K+ channel 1, K2P13.1

Short Description :

A Rabbit Polyclonal Antibody to KCNK13 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope in the 1st extracellular loop of the rat KCNK13 channel. Anti-KCNK13 (THIK-1) (extracellular) Antibody (#APC-121) can be used in western blot and immunohistochemical applications, and recognizes KCNK13 channel from mouse and rat samples.

Negative Control :

BLP-PC121

Positive Control :

Synonyms :

Potassium channel subfamily K member 13, Tandem pore domain halothane-inhibited K+ channel 1, K2P13.1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNK13

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50-1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage