Anti-KV4.1 (KCND1) Antibody | Alomone Labs APC-119 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV4.1 (KCND1) Antibody

catalog :

APC-119

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Kim K, Lee S, Yoon S, Kim Y, Jeong H, Lee S, et al. Kv4.1, a Key Ion Channel For Low Frequency Firing of Dentate Granule Cells, Is Crucial for Pattern Separation. J Neurosci. 2020;40:2200-2214 pubmed publisher
Wolff M, Czorlich P, Nagaraj C, SchnÃ¶bel Ehehalt R, Li Y, Kwapiszewska G, et al. Amitriptyline and carbamazepine utilize voltage-gated ion channel suppression to impair excitability of sensory dorsal horn neurons in thin tissue slice: An in vitro study. Neurosci Res. 2016;109:16-27 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-KV4.1 (KCND1) Antibody (#APC-119), (1:400).2. Anti- KV4.1 (KCND1) Antibody, preincubated with Kv4.1/KCND1 Blocking Peptide (#BLP-PC119).

image 2 :

Western blot analysisof rat brain lysate: - 1.Anti-KV4.1(KCND1)Antibody (#APC-119) (1:400).2. Anti- KV4.1 (KCND1) Antibody preincubated with the negative control antigen.

image 3 :

Peptide (C)KRRAIRLANSTAS corresponding to amino acid residues 538-550 of human KCND1(AccessionQ9NSA2). Intracellular C-terminal domain.

product information

CAT :

APC-119

SKU :

APC-119-CF_0.2 ml

Product Name :

Anti-KV4.1 (KCND1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9NSA2

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC119

Homology :

Mouse, rat - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KRRAIRLANSTAS, corresponding to amino acid residues 538-550 of human KCND1

Product Page - Scientific background :

KV4.1 is a voltage-dependent K+ channel that belongs to the Shal channel subfamily and includes two other members: KV4.2 and KV4.3.KV4.1 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C-termini. As with other members of the voltage-gated K+ channel superfamily, the functional channel is a tetramer that can be composed of more than one member of the Shal subfamily, i.e. heterotetramers of KV4.1 and KV4.2.The KV4 channels are characterized by activation at subthreshold membrane potentials, inactivate rapidly and recover from inactivation quickly compared with other voltage-dependent K+ channels. This type of current is known as transient A-type K+ currents. For example, depolarization-activated K+ currents in rat neostriatal cholinergic interneurons are predominantly of the A-type and attributable to coexpression of KV4.1 and KV4.2 subunits. The biophysical properties of the KV4.1 subunit can be modified by its association with auxiliary b subunits such as KChIP1 that increase KV4.1 current densities and accelerates both the inactivation and the recovery time.KV4.1 is highly expressed in the brain but is also expressed in peripheral tissues such as the colon, heart and lung.The pharmacology of KV4.1 has been less well studied than that of the other KV4 members. KV4.1 is sensitive to the classical K+ channel blockers, 4-aminopyridine and tetraethylammonium. Recently, HmTx1, a peptide blocker derived from the venom of the African tarantula Heteroscodra maculata, has been shown to be a potent KV4.1 inhibitor.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Voltage-gated potassium channel subfamily D member 1

Short Description :

A Rabbit Polyclonal Antibody to KV4.1 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of human KV4.1. The Anti-KV4.1 (KCND1) Antibody (#APC-119) can be used for western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KV4.1 from rat, human, and mouse samples.

Negative Control :

BLP-PC119

Positive Control :

Synonyms :

Voltage-gated potassium channel subfamily D member 1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCND1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:400

more info or order :

Alomone Labs product webpage