Anti-KCNE3 (MiRP2) Antibody | Alomone Labs APC-118 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNE3 (MiRP2) Antibody

catalog :

APC-118

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Inagaki A, Hayashi M, Andharia N, Matsuda H. Involvement of butyrate in electrogenic K+ secretion in rat rectal colon. Pflugers Arch. 2019;471:313-327 pubmed publisher
King E, Patel V, Anand M, Zhao X, Crump S, Hu Z, et al. Targeted deletion of Kcne3 impairs skeletal muscle function in mice. FASEB J. 2017;31:2937-2947 pubmed publisher

image

image 1 :

Western blot analysis of rat kidney (lanes 1 and 3) and heart (lanes 2 and 4) membranes: - 1,2. Anti-KCNE3 (MiRP2) Antibody (#APC-118), (1:200).3,4. Anti-KCNE3 (MiRP2) Antibody, preincubated with KCNE3/MiRP2 Blocking Peptide (#BLP-PC118).

image 2 :

Peptide (C)RSRKVDKRSDPYH corresponding to amino acid residues 81-93 of human KCNE3 (AccessionQ9Y6H6). Intracellular C-terminal part.

image 3 :

Western blot analysis of human U-87 MG glioblastoma cell line lysate: - 1. Anti-Slitrk2 (extracellular) Antibody (#ASR-082) (1:200).2. Anti-Slitrk2 (extracellular) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-118

SKU :

APC-118-CF_0.2 ml

Product Name :

Anti-KCNE3 (MiRP2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9Y6H6

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC118

Homology :

Rat, mouse, dog, pig - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RSRKVDKRSDPYH, corresponding to amino acid residues 81-93 of human KCNE3

Product Page - Scientific background :

KCNE3 (or MiRP2) is a member of a family of proteins that regulate the activity of voltage-dependent K+ channels. Other members of the family are KCNE1 (IsK, MiNK), KCNE2 (MiRP1), KCNE4 (MiRP3) and KCNE5 (MiRP4).KCNE1 is the founding member of the family and was initially believed to form a K+ channel itself, but was later recognized to work as a regulatory b subunit associated with the KV7.1 (KCNQ1) a protein. KCNE3 was discovered based on its homology with KCNE1.The KCNE regulatory subunits are small proteins (14-20 kD) with a type-1 integral membrane topology. It is believed that both the cytoplasmic C-terminus tail and the transmembrane domain are necessary for the interaction with the a subunits. The stoichiometry of the KCNE subunits with their partner a subunits in the native channels is not clear and ratios ranging from 2 to 14 KCNE subunits per 4 a subunits have been proposed.KCNE3 is relatively widely expressed in several tissues with prominent expression in the kidney and skeletal muscle.KCNE3 is quite promiscuous and associations with KV7.1, KV3.4, KV7.4 (KCNQ4), KV11.1 (HERG), KV2.1 and KV3.1b have been demonstrated. The best characterized interactions are with the former two proteins. KCNE3 interacts with KV7.1 in epithelial cells of the gastrointestinal tract where it appears to be important for Na+ absorption. In skeletal muscle, KCNE3 couples to KV3.4 to regulate muscle function. Indeed, a mutation in KCNE3 (R83H) has been associated with an inherited form of periodic paralysis (Thyrotoxic hypokalemic periodic paralysis).

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Potassium channel subunit β MiRP2, MinK-related peptide 2, Potassium voltage-gated channel subfamily E member 3, HOKPP

Short Description :

A Rabbit Polyclonal Antibody to KCNE3 (MiRP2) Subunit

Long Description :

Anti-KCNE3 (MiRP2) Antibody (#APC-118) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunocytochemistry and immunohistochemistry applications. It has been designed to recognize KCNE3 from rat, mouse, and human samples.

Negative Control :

BLP-PC118

Positive Control :

Synonyms :

Potassium channel subunit β MiRP2, MinK-related peptide 2, Potassium voltage-gated channel subfamily E member 3, HOKPP

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNE3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage