Anti-KVβ2 Antibody | Alomone Labs APC-117 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KVβ2 Antibody

catalog :

APC-117

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and membranes (lanes 2 and 4): - 1,2. Anti-KVβ2 Antibody (#APC-117), (1:200).3,4. Anti-KVβ2 Antibody, preincubated with Kvβ2 Blocking Peptide (#BLP-PC117).

image 2 :

Expression of KVβ2 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum using Anti-KVβ2 Antibody (#APC-117). A. KVβ2 appears adjacent to Purkinje cells (green). B. Staining of GABAergic cells with mouse anti-parvalbumin (PV, red). C. Confocal merge of KVβ2 and PV demonstrates the presence of KVβ2 adjacent to Purkinje cells.

image 3 :

Western blot analysisof human Jurkat T cells: - 1.Anti-KV?2 Antibody (#APC-117)(1:200).2. Anti-KV?2 Antibody preincubated with the negative control antigen.

product information

CAT :

APC-117

SKU :

APC-117-CF_0.2 ml

Product Name :

Anti-KVβ2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P62483

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC117

Homology :

Mouse, human, rabbit - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

N-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

SPGMIYSTRYGSPKR(C), corresponding to amino acid residues 20-34 of rat KCNAB2

Product Page - Scientific background :

KVβ2 (or KCNAB2) is a member of a family of proteins that regulate the activity of voltage-dependent K+ channels. The other members of the family are KVβ1 and KVβ3.The KVβ subunits were originally identified using a biochemical approach that demonstrated that the ion-conducting α subunits existed in a macromolecular complex with auxiliary β subunits with a probable stoichiometry of α4β4. It is now widely established that the regulatory β subunits are able to alter both the biophysical (i.e. acceleration of inactivation kinetics) and biochemical (promote cell surface expression) properties of the functional KV channel.The KVβ regulatory subunits are cytosolic proteins with conserved C-termini and variable N-terminus domains. The interaction with the α subunits is via the conserved C-terminus that binds to a specific sequence in the N-terminus of the α subunits.KVβ2, as well as the other KVβ subunits can bind to all the members of the large KV1.x (Shaker) channel subfamily. However, recent evidence suggests that it can also bind to members of the KV4.x subfamily.KVβ2 is the most abundant β subunit in the brain, where it couples with all the KV1.x subunits, but the protein is also expressed in peripheral tissues including the heart, lungs and cells of hematopoietic origin such as T cells where it couples with KV1.3, the dominant voltage-dependent K+ channel in these cells.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Voltage-gated potassium channel subunit beta-2, KV beta 2, KCNAB2, Ckbeta2

Short Description :

A Rabbit Polyclonal Antibody to KV?2 Subunit

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat KVβ2. Anti-KVβ2 Antibody (#APC-117) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize KVβ2 from rat, mouse and human samples.

Negative Control :

BLP-PC117

Positive Control :

Synonyms :

Voltage-gated potassium channel subunit beta-2, KV beta 2, KCNAB2, Ckbeta2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNAB2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage