Anti-KV12.3 (KCNH4) Antibody | Alomone Labs APC-116 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV12.3 (KCNH4) Antibody

catalog :

APC-116

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

image

image 1 :

Western blot analysis of rat brain (lanes 1 and 3) and cerebellum (lanes 2 and 4) lysates: - 1,2. Anti-KV12.3 (KCNH4) Antibody (#APC-116), (1:200).3,4. Anti-KV12.3 (KCNH4) Antibody, preincubated with Kv12.3/KCNH4 Blocking Peptide (#BLP-PC116).

image 2 :

Expression of KV12.3 (Elk3) in rat testes - Immunohistochemical staining of rat testes paraffin embedded section using Anti-KV12.3 (KCNH4) Antibody (#APC-116), (1:100).KV12.3 (pink staining) is expressed in the columnar epithelium of the epididymus. Hematoxilin is used as the counterstain.

image 3 :

Expression of KV12.3 (Elk3) in rat testes - Immunohistochemical staining of rat testes paraffin embedded section usingAnti-KV12.3 (KCNH4)Antibody (#APC-116) (1:100).KV12.3 (pink staining) is expressed in the columnar epithelium of the epididymus. Hematoxilin is used as the counterstain.

product information

CAT :

APC-116

SKU :

APC-116-CF_0.2 ml

Product Name :

Anti-KV12.3 (KCNH4) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9R1T9

Applications :

IHC WB

Reactivity :

Human Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC116

Homology :

Human - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CRLNQEISRLNQEVS, corresponding to amino acids 881-895 of rat KCNH4

Product Page - Scientific background :

KV12.3 (also known as KCNH4, Elk3) is a voltage-gated K+ channel that belongs to the ether-a-go-go (EAG) family. The EAG family of voltage-gated K+ channels can be subdivided into three distinct groups based on sequence homology. These are the eag (KV10) with two members, the eag-related channels (erg or KV11) and the eag-like K+ channels (elk or KV12) with three members each.As with all members of the family, KV12.3 possesses the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C- termini. The functional channel is a tetramer composed of four subunits. It has been suggested that the KV12 subfamily members can form functional heteromultimers within the subfamily.1,2KV12.3 channel distribution appears to be mainly confined to the central nervous system with some expression in peripheral organs such as the testis and lung.3The physiological function of the KV12.3 channel is yet to be established, although a role in the modulation of overall excitability of neurons has been suggested.3 Currently, there isn't much pharmacological data concerning the modulation of the KV12.3 channel except for blocking of the channel by Ba2+.3

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily H member 4, Ether-a-go-go-like potassium channel 1, ELK channel 1, rElk1, Elk3, Brain-specific eag-like channel 2, BEC2

Short Description :

A Rabbit Polyclonal Antibody to KV12.3 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope located at the intracellular C-terminal end of the rat KV12.3. Anti-KV12.3 (KCNH4) Antibody (#APC-116) can be used in western blot and immunohistochemical applications, and recognizes KV12.3 from rat and human samples.

Negative Control :

BLP-PC116

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily H member 4, Ether-a-go-go-like potassium channel 1, ELK channel 1, rElk1, Elk3, Brain-specific eag-like channel 2, BEC2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNH4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IFC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage