Anti-KCNH6 (erg2) Antibody | Alomone Labs APC-114 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNH6 (erg2) Antibody

catalog :

APC-114

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Holt J, Jordan P, Lysakowski A, Shah A, Barsz K, Contini D. Muscarinic Acetylcholine Receptors and M-Currents Underlie Efferent-Mediated Slow Excitation in Calyx-Bearing Vestibular Afferents. J Neurosci. 2017;37:1873-1887 pubmed publisher

image

image 1 :

Western blot analysis of rat brain (1 and 3) and cortex (2 and 4) lysate: - 1,2. Anti-KCNH6 (erg2) Antibody (#APC-114), (1:200).3,4. Anti-KCNH6 (erg2) Antibody, preincubated with KCNH6/erg2 Blocking Peptide (#BLP-PC114).

image 2 :

Expression of KV11.2 (erg2) in rat hippocampus - Immunohistochemical staining of KV11.2 in rat hippocampus using Anti-KCNH6 (erg2) Antibody (#APC-114). A. KV11.2 appears as diffuse staining (green) that defines the boundary of the reticular nucleus of thalamus (arrow). B. Staining with mouse anti-parvalbumin (PV, red) demonstrates both neuronal and diffuse staining. C. Confocal merge of KV11.2 and PV demonstrates overlap of the two markers labeling the reticular nucleus of the thalamus (orange).

image 3 :

Expression of KV11.2 (erg2) in rat hippocampus - Immunohistochemical staining of KV11.2 in rat hippocampus usingAnti-KCNH6 (erg2) Antibody(#APC-114). A. KV11.2 appears as diffuse staining (green) that defines the boundary of the reticular nucleus of thalamus (arrow). B. Staining with mouse anti-parvalbumin (PV red) demonstrates both neuronal and diffuse staining. C. Confocal merge of KV11.2 and PV demonstrates overlap of the two markers labeling the reticular nucleus of the thalamus (orange).

product information

CAT :

APC-114

SKU :

APC-114-CF_0.2 ml

Product Name :

Anti-KCNH6 (erg2) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H252

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC114

Homology :

Rat, chicken - identical; mouse - 13/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, N-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

TLNFVEFNLEKHRS(C), corresponding to amino acid residues 185-198 of human KV11.2 (erg2)

Product Page - Scientific background :

KV11.2 (erg2) is a member of the ether-a-go-go (EAG) subfamily of voltage-dependent K+ channels. The erg subfamily includes the closely related proteins KV11.1 (erg1) and KV11.3 (erg3) that possess the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C-termini. As with all voltage-dependent K+ channels, the functional channel is a tetramer composed of four subunits. It has been suggested that the KV11 subfamily members can form functional heteromultimers within the subfamily.KV11.2 produces currents characterized by strong inward rectification with slow activation and very rapid inactivation kinetics, which closely resemble those produced by the much studied channel KV11.1.The expression of KV11.2 seems to be limited to the brain and the pituitary gland. The same is true for the KV11.3 protein, while KV11.1 is more widely expressed. From a pharmacological point of view, KV11.2 channels can be blocked by well characterized organic blockers such as the anti-arrhythmic drug E-4031. In addition, the peptide toxins Ergtoxin-1 and BeKm-1 block KV11.2 with different potencies.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

KV11.2, Potassium voltage-gated channel subfamily H member 6, Ether-a-go-go-related channel 2, Eag-related protein 2, ERG-2, hERG2

Short Description :

A Rabbit Polyclonal Antibody to KV11.2 (ERG-2) Channel

Long Description :

Alomone Labs is pleased to offer Anti-KCNH6 (erg2) Antibody (#APC-114) directed against a highly conserved epitope of the human KV11.2 channel. The antibody can be used to detect KV11.2 in rat samples using both western blotting and immunohistochemistry applications.

Negative Control :

BLP-PC114

Positive Control :

Synonyms :

KV11.2, Potassium voltage-gated channel subfamily H member 6, Ether-a-go-go-related channel 2, Eag-related protein 2, ERG-2, hERG2

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNH6

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage