Anti-KCNH7 (erg3) Antibody | Alomone Labs APC-112 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNH7 (erg3) Antibody

catalog :

APC-112

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 2

Reference
Xiao K, Sun Z, Jin X, Ma W, Song Y, Lai S, et al. ERG3 potassium channel-mediated suppression of neuronal intrinsic excitability and prevention of seizure generation in mice. J Physiol. 2018;596:4729-4752 pubmed publisher
Holt J, Jordan P, Lysakowski A, Shah A, Barsz K, Contini D. Muscarinic Acetylcholine Receptors and M-Currents Underlie Efferent-Mediated Slow Excitation in Calyx-Bearing Vestibular Afferents. J Neurosci. 2017;37:1873-1887 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-KCNH7 (erg3) Antibody (#APC-112), (1:300).2. Anti-KCNH7 (erg3) Antibody, preincubated with KCNH7/erg3 Blocking Peptide (#BLP-PC112).

image 2 :

Expression of KV11.3 (erg3) in rat cerebellum - Immunohistochemical staining of rat cerebellum using Anti-KCNH7 (erg3) Antibody (#APC-112). A. KV11.3 channel appears in glial processes (red). B. Staining of astrocytic fibers with mouse anti-glial fibrillary acidic protein (GFAP, green). C. Confocal merge of KV11.3 channel and GFAP demonstrates colocalization in the molecular layer but separate localization of these proteins at the Purkinje cell layer.

image 3 :

Expression of KV11.3 (erg3) in rat cerebellum - Immunohistochemical staining of rat cerebellum usingAnti-KCNH7 (erg3) Antibody(#APC-112). A. KV11.3 channel appears in glial processes (red). B. Staining of astrocytic fibers with mouse anti-glial fibrillary acidic protein (GFAP green). C. Confocal merge of KV11.3 channel and GFAP demonstrates colocalization in the molecular layer but separate localization of these proteins at the Purkinje cell layer.

product information

CAT :

APC-112

SKU :

APC-112-CF_0.2 ml

Product Name :

Anti-KCNH7 (erg3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O54852

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC112

Homology :

Human - identical; mouse - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

CPEFLDLEKSKLKSKE, corresponding to amino acid residues 1108-1123 of rat KV11.3 (erg3)

Product Page - Scientific background :

KV11.3 (erg3) is a member of the ether-a-go-go (EAG) subfamily of voltage-dependent K+ channels. The erg subfamily includes the closely related proteins KV11.1 (erg1) and KV11.2 (erg2) that possess the signature structure of the voltage-dependent K+ channels: six membrane-spanning domains with intracellular N- and C-termini. As with all voltage-dependent K+ channels, the functional channel is a tetramer composed of four subunits. It has been suggested that the KV11 subfamily members can form functional heteromultimers within the subfamily.The current of the KV11.1 and KV11.2 channels is that of a strong inward rectifier with slow activating kinetics. The KV11.3 channel has different biophysical properties: it is a weak inward rectifier that is activated at negative potentials and has rapid activating kinetics.KV11.3 expression is believed to be concentrated mainly in the brain with the stronger expression detected in the cerebral cortex and hippocampus.Currently, there is not much pharmacological information concerning KV11.3 modulation, but the channel has been shown to be inhibited (as are all KV11 subfamily members) by the anti-arrhythmic E-4031 as well as by the anti-psychotic drugs sertindole and pimozide.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

KV11.3, Potassium voltage-gated channel subfamily H member 7, Ether-a-go-go-related channel 3, Eag-related protein 3, ERG-3, hERG3

Short Description :

A Rabbit Polyclonal Antibody to KV11.3 (ERG-3) Channel

Long Description :

Alomone Labs is pleased to offer Anti-KCNH7 (erg3) Antibody (#APC-112) that is directed against a highly conserved epitope of the rat KV11.3 channel. The antibody will also react with human and mouse samples. The antibody can be used in western blot, immunohistochemistry and immunocytochemistry applications.

Negative Control :

BLP-PC112

Positive Control :

Synonyms :

KV11.3, Potassium voltage-gated channel subfamily H member 7, Ether-a-go-go-related channel 3, Eag-related protein 3, ERG-3, hERG3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNH7

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:300

more info or order :

Alomone Labs product webpage