Anti-KCNK1 (TWIK-1) (extracellular) Antibody | Alomone Labs APC-110 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNK1 (TWIK-1) (extracellular) Antibody

catalog :

APC-110

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 3

Reference
Fischer R, Roux A, Wareham L, Sappington R. Pressure-dependent modulation of inward-rectifying K+ channels: implications for cation homeostasis and K+ dynamics in glaucoma. Am J Physiol Cell Physiol. 2019;317:C375-C389 pubmed publisher
Thompson K, Chen J, Luo Q, Xiao Y, Cummins T, Bhatwadekar A. Advanced glycation end (AGE) product modification of laminin downregulates Kir4.1 in retinal Müller cells. PLoS ONE. 2018;13:e0193280 pubmed publisher
Mao Q, Yuan J, Ming X, Wu S, Chen L, Bekker A, et al. Role of dorsal root ganglion K2p1.1 in peripheral nerve injury-induced neuropathic pain. Mol Pain. 2017;13:1744806917701135 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate: - 1. Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110), (1:200).2. Anti-KCNK1 (TWIK-1) (extracellular) Antibody, preincubated with KCNK1/TWIK-1 (extracellular) Blocking Peptide (#BLP-PC110).

image 2 :

Expression of KCNK1 in mouse cerebellum - Immunohistochemical staining of mouse cerebellum using Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110). A. KCNK1 channel appears in glial processes (red). B. Staining of Purkinje nerve cells with mouse anti-calbindin D28K (a calcium binding protein, green). C. Confocal merge of KCNK1 channel and calbindin D28K demonstrates the separate localization of these proteins.

image 3 :

Western blot analysisof HEK-293-KCNK1transfected cells: - 1.Anti-KCNK1(TWIK-1) (extracellular) Antibody (#APC-110)(1:300).2. Anti-KCNK1(TWIK-1) (extracellular) Antibody preincubated with the control peptide antigen.

product information

CAT :

APC-110

SKU :

APC-110-CF_0.2 ml

Product Name :

Anti-KCNK1 (TWIK-1) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

O00180

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC110

Homology :

Rat, mouse, pig - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular, near the P1 loop

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

RQELRKLKRRFLEEHEC, corresponding to amino acid residues 53-69 of human KCNK1

Product Page - Scientific background :

KCNK1 (also named TWIK-1 or K2P1.1) is a member of the 2-pore (2P) domain K+ channels family that, currently, includes 16 members. These channels show little time- or voltage-dependence and are considered to be "leak" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C termini. K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. but are insensitive to the classical K+ channel blockers.KCNK1 was the first of the K2P channels to be identified and as its original name indicates (Tandem of P domains in a Weak Inward rectifier K+ channel) the channel behaves as a weak inward rectifier when expressed in heterologous systems. The channel is ubiquitously expressed with the most prominent expression in the brain, kidney and heart.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Potassium channel subfamily K member 1, Inward rectifying K+ channel protein TWIK-1, K2P1.1, HOHO1, KCNO1

Short Description :

A Rabbit Polyclonal Antibody to KCNK1 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the human K2P1.1 channel. Anti-KCNK1 (TWIK-1) (extracellular) Antibody (#APC-110) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting KCNK1 in living cells. It has been designed to recognize KCNK1 from mouse, rat and human samples.

Negative Control :

BLP-PC110

Positive Control :

Synonyms :

Potassium channel subfamily K member 1, Inward rectifying K+ channel protein TWIK-1, K2P1.1, HOHO1, KCNO1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNK1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:300

more info or order :

Alomone Labs product webpage