Anti-KCNK4 (TRAAK) Antibody | Alomone Labs APC-108 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNK4 (TRAAK) Antibody

catalog :

APC-108

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Wang L, Shi K, Li H, Huang H, Wu W, Cai C, et al. Activation of the TRAAK two-pore domain potassium channels in rd1 mice protects photoreceptor cells from apoptosis. Int J Ophthalmol. 2019;12:1243-1249 pubmed publisher
Zhang X, Xu Z, Shi K, Guo D, Li H, Wang L, et al. Elevated expression of TREK-TRAAK K2P channels in the retina of adult rd1 mice. Int J Ophthalmol. 2019;12:924-929 pubmed publisher
Fischer R, Roux A, Wareham L, Sappington R. Pressure-dependent modulation of inward-rectifying K+ channels: implications for cation homeostasis and K+ dynamics in glaucoma. Am J Physiol Cell Physiol. 2019;317:C375-C389 pubmed publisher
Viatchenko Karpinski V, Ling J, Gu J. Characterization of temperature-sensitive leak K+ currents and expression of TRAAK, TREK-1, and TREK2 channels in dorsal root ganglion neurons of rats. Mol Brain. 2018;11:40 pubmed publisher
Shen C, Ma W, Zheng W, Huang H, Xia R, Li C, et al. The antioxidant effects of riluzole on the APRE-19 celll model injury-induced by t-BHP. BMC Ophthalmol. 2017;17:210 pubmed publisher

image

image 1 :

Western blot analysis of HEK-293-KCNK4 transfected cells: - 1. Anti-KCNK4 (TRAAK) Antibody (#APC-108), (1:200).2. Anti-KCNK4 (TRAAK) Antibody, preincubated with KCNK4/TRAAK Blocking Peptide (#BLP-PC108).

image 2 :

Expression of KCNK4 in rat cerebellum - Immunohistochemical staining of rat cerebellum using Anti-KCNK4 (TRAAK) Antibody (#APC-108 ). A. KCNK4 channel appears in Purkinje neuronal processes (red). B. Staining of Purkinje nerve cells with mouse anti-calbindin D28K (a calcium binding protein, green). C. Confocal merge of KCNK4 channel and calbindin D28K demonstrates the co-localization of these proteins.

image 3 :

Western blot analysisof HEK-293-KCNK4transfected cells: - 1.Anti-KCNK4(TRAAK)Antibody (#APC-108)(1:200).2. Anti-KCNK4 (TRAAK) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-108

SKU :

APC-108-CF_0.2 ml

Product Name :

Anti-KCNK4 (TRAAK) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9NYG8

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC108

Homology :

Rat, mouse, dog - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

NLAFIDESSDTQSERGC, corresponding to amino acid residues 343-359 of human KCNK4

Product Page - Scientific background :

KCNK4 (also named TWIK-related arachidonic acid stimulated K+ channel, TRAAK or K2P4.1) is a member of the 2-pore (2P) domain K+ channels family that at the moment includes 16 members. These channels show little time- or voltage-dependence and are considered to be "leaky" or "background" K+ channels, thereby generating background currents which help set the membrane resting potential and cell excitation. The K2P channels have a signature topology that includes four transmembrane domains and two pore domains with intracellular N- and C- termini.K2P channels are regulated by diverse physical and chemical stimuli including temperature, pH, mechanical stretch, inhalation anesthetics, etc. The channels can then be subclassified based in their specific activators. KCNK4 can be integrated to a K2P subfamily that includes K2P2.1 (TREK1) and K2P10.1 (TREK2) that are activated by intracellular unsaturated fatty acids such as arachidonic acid, lysophosphatidic acid, high intracellular pH and mechanical stretch.KCNK4 expression is largely confined to the brain in mouse samples while in humans it is also expressed in placenta and to a lesser degree in kidney, small intestine and prostate.

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Potassium channel subfamily K member 4, K2P4.1, TWIK-related arachidonic acid-stimulated K+ channel protein, Two pore K+ channel KT4.1

Short Description :

A Rabbit Polyclonal Antibody to KCNK4 Channel

Long Description :

Anti-KCNK4 (TRAAK) Antibody (#APC-108) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KCNK4 from rat, mouse, and human samples.

Negative Control :

BLP-PC108

Positive Control :

Synonyms :

Potassium channel subfamily K member 4, K2P4.1, TWIK-related arachidonic acid-stimulated K+ channel protein, Two pore K+ channel KT4.1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNK4

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage