Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody | Alomone Labs APC-107 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody

catalog :

APC-107

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunohistochemistry - frozen section, proximity ligation assay

more info or order :

Alomone Labs product webpage

citations: 29

Published Application/Species/Sample/Dilution	Reference
immunohistochemistry; rat; loading ...; fig 1c western blot; rat; loading ...; fig 2d	Yang L, Han B, Zhang M, Wang Y, Tao K, Zhu M, et al. Activation of BK Channels Prevents Hepatic Stellate Cell Activation and Liver Fibrosis Through the Suppression of TGFβ1/SMAD3 and JAK/STAT3 Profibrotic Signaling Pathways. Front Pharmacol. 2020;11:165 pubmed publisher
western blot; human; 1:200; loading ...; fig 7g	Kicinska A, Augustynek B, Kulawiak B, Jarmuszkiewicz W, Szewczyk A, Bednarczyk P. A large-conductance calcium-regulated K+ channel in human dermal fibroblast mitochondria. Biochem J. 2016;473:4457-4471 pubmed
western blot; human; 1:1000; fig 6b	Cheng J, Mao L, Wen J, Li P, Wang N, Tan X, et al. Different Effects of Hypertension and Age on the Function of Large Conductance Calcium- and Voltage-Activated Potassium Channels in Human Mesentery Artery Smooth Muscle Cells. J Am Heart Assoc. 2016;5: pubmed publisher
immunohistochemistry - frozen section; mouse; 1:200; fig 5	Li Y, Hu H, Butterworth M, Tian J, Zhu M, O Neil R. Expression of a Diverse Array of Ca2+-Activated K+ Channels (SK1/3, IK1, BK) that Functionally Couple to the Mechanosensitive TRPV4 Channel in the Collecting Duct System of Kidney. PLoS ONE. 2016;11:e0155006 pubmed publisher
western blot; human; 1:500; fig 3	Edalat L, Stegen B, Klumpp L, Haehl E, Schilbach K, Lukowski R, et al. BK K+ channel blockade inhibits radiation-induced migration/brain infiltration of glioblastoma cells. Oncotarget. 2016;7:14259-78 pubmed publisher
proximity ligation assay; rat; fig 4b western blot; rat; fig 4a	Chen M, Li J, Jiang F, Fu J, Xia X, Du J, et al. Orai1 forms a signal complex with BKCa channel in mesenteric artery smooth muscle cells. Physiol Rep. 2016;4: pubmed publisher
	Ferreira J, Amazu C, Puga Molina L, Ma X, England S, Santi C. SLO2.1/NALCN a sodium signaling complex that regulates uterine activity. iScience. 2021;24:103210 pubmed publisher
	Ohya S, Kajikuri J, Endo K, Kito H, Elboray E, Suzuki T. Ca2+ -activated K+ channel KCa 1.1 as a therapeutic target to overcome chemoresistance in three-dimensional sarcoma spheroid models. Cancer Sci. 2021;112:3769-3783 pubmed publisher
	Pinilla E, S xe1 nchez A, Mart xed nez M, Mu xf1 oz M, Garc xed a Sacrist xe1 n A, K xf6 hler R, et al. Endothelial KCa 1.1 and KCa 3.1 channels mediate rat intrarenal artery endothelium-derived hyperpolarization response. Acta Physiol (Oxf). 2021;231:e13598 pubmed publisher
	Xu X, Li Y, Wu Y, Xu Y, Weng R, Wang C, et al. Upregulation of spinal ASIC1 by miR-485 mediates enterodynia in adult offspring rats with prenatal maternal stress. CNS Neurosci Ther. 2021;27:244-255 pubmed publisher
	Gu F, Parada I, Yang T, Longo F, Prince D. Partial Activation of TrkB Receptors Corrects Interneuronal Calcium Channel Dysfunction and Reduces Epileptogenic Activity in Neocortex following Injury. Cereb Cortex. 2020;: pubmed publisher
	Shrum S, Tobacyk J, Lo S, Parajuli N, Macmillan Crow L. The BK activator NS11021 partially protects rat kidneys from cold storage and transplantation-induced mitochondrial and renal injury. Arch Biochem Biophys. 2020;688:108410 pubmed publisher
	Fan F, Geurts A, Pabbidi M, Ge Y, Zhang C, Wang S, et al. A Mutation in γ-Adducin Impairs Autoregulation of Renal Blood Flow and Promotes the Development of Kidney Disease. J Am Soc Nephrol. 2020;31:687-700 pubmed publisher
	Shrum S, Rusch N, Macmillan Crow L. Specific BK Channel Activator NS11021 Protects Rat Renal Proximal Tubular Cells from Cold Storage-Induced Mitochondrial Injury In Vitro. Biomolecules. 2019;9: pubmed publisher
	Noda S, Suzuki Y, Yamamura H, Giles W, Imaizumi Y. Roles of LRRC26 as an auxiliary γ1-subunit of large-conductance Ca2+-activated K+ channels in bronchial smooth muscle cells. Am J Physiol Lung Cell Mol Physiol. 2020;318:L366-L375 pubmed publisher
	Wang Y, Guo Q, Hei H, Tao J, Zhou Y, Dong J, et al. BK ablation attenuates osteoblast bone formation via integrin pathway. Cell Death Dis. 2019;10:738 pubmed publisher
	Saeki T, Suzuki Y, Yamamura H, Takeshima H, Imaizumi Y. A junctophilin-caveolin interaction enables efficient coupling between ryanodine receptors and BKCa channels in the Ca2+ microdomain of vascular smooth muscle. J Biol Chem. 2019;294:13093-13105 pubmed publisher
	Wang Y, Tao J, Wang M, Yang L, Ning F, Xin H, et al. Mechanism of Regulation of Big-Conductance Ca2+-Activated K+ Channels by mTOR Complex 2 in Podocytes. Front Physiol. 2019;10:167 pubmed publisher
	Chubinskiy Nadezhdin V, Sudarikova A, Shilina M, Vasileva V, Grinchuk T, Lyublinskaya O, et al. Cell Cycle-Dependent Expression of Bk Channels in Human Mesenchymal Endometrial Stem Cells. Sci Rep. 2019;9:4595 pubmed publisher
	Bergsma A, Ganguly S, Wiegand M, Dick D, Williams B, Miranti C. Regulation of cytoskeleton and adhesion signaling in osteoclasts by tetraspanin CD82. Bone Rep. 2019;10:100196 pubmed publisher
	Zhang J, Guan X, Li Q, Meredith A, Pan H, Yan J. Glutamate-activated BK channel complexes formed with NMDA receptors. Proc Natl Acad Sci U S A. 2018;115:E9006-E9014 pubmed publisher
	Lu M, Li J, Alvarez Lugo L, Li Y, Yu S, Li X, et al. Lipopolysaccharide stimulates BK channel activity in bladder umbrella cells. Am J Physiol Cell Physiol. 2018;314:C643-C653 pubmed publisher
	Liu W, Löwenheim H, Santi P, Glueckert R, Schrott Fischer A, Rask Andersen H. Expression of trans-membrane serine protease 3 (TMPRSS3) in the human organ of Corti. Cell Tissue Res. 2018;372:445-456 pubmed publisher
	Nieves Cintrón M, Syed A, Buonarati O, Rigor R, Nystoriak M, Ghosh D, et al. Impaired BKCa channel function in native vascular smooth muscle from humans with type 2 diabetes. Sci Rep. 2017;7:14058 pubmed publisher
	Comerma Steffensen S, Carvacho I, Hedegaard E, Simonsen U. Small and Intermediate Calcium-Activated Potassium Channel Openers Improve Rat Endothelial and Erectile Function. Front Pharmacol. 2017;8:660 pubmed publisher
	Velázquez Marrero C, Burgos A, García J, Palacio S, Marrero H, Bernardo A, et al. Alcohol Regulates BK Surface Expression via Wnt/?-Catenin Signaling. J Neurosci. 2016;36:10625-10639 pubmed
	Hei H, Gao J, Dong J, Tao J, Tian L, Pan W, et al. BK Knockout by TALEN-Mediated Gene Targeting in Osteoblasts: KCNMA1 Determines the Proliferation and Differentiation of Osteoblasts. Mol Cells. 2016;39:530-5 pubmed publisher
	Bhattarai Y, Fried D, Gulbransen B, Kadrofske M, Fernandes R, Xu H, et al. High-fat diet-induced obesity alters nitric oxide-mediated neuromuscular transmission and smooth muscle excitability in the mouse distal colon. Am J Physiol Gastrointest Liver Physiol. 2016;311:G210-20 pubmed publisher
	Mizutani H, Yamamura H, Muramatsu M, Hagihara Y, Suzuki Y, Imaizumi Y. Modulation of Ca2+ oscillation and melatonin secretion by BKCa channel activity in rat pinealocytes. Am J Physiol Cell Physiol. 2016;310:C740-7 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107), (1:500). 2. Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody, preincubated with KCNMA1/KCa1.1 (1184-1200) Blocking Peptide (#BLP-PC107).

image 2 :

Expression of KCNMA1 in rat penis - Immunohistochemical staining of rat penis transversal section using Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107). Strong and specific immunostaining is evident in both corpus cavernosum smooth muscle cells (blue arrow) and in the muscular layer of the penis artery (green arrow). Universal Immuno-alkaline-phosphatase Polymer followed by New Fuchsin Subtrate (histofine, Nichirei Corp.) was used for the colour reaction. Hematoxillin is used as the counterstain.

image 3 :

Expression of KCNMA1in rat penis - Immunohistochemical staining of rat penis transversal sectionusingAnti-KCNMA1 (KCa1.1) (1184-1200)Antibody (#APC-107). Strong and specific immunostaining is evident in both corpus cavernosum smooth muscle cells (blue arrow) and in the muscular layer of the penis artery (green arrow). Universal Immuno-alkaline-phosphatase Polymer followed by New Fuchsin Subtrate (histofine Nichirei Corp.) was used for the colour reaction. Hematoxillin is used as the counterstain.

product information

CAT :

APC-107

SKU :

APC-107-CF_0.2 ml

Product Name :

Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q08460

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC107

Homology :

Rat - identical; human, bovine, chicken, dog - 16/17 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Mouse

Sequence :

(C)STANRPNRPKSRESRDK, corresponding to amino acid residues 1184-1200 of mouse KCNMA1

Product Page - Scientific background :

KCa1.1 (KCNMA1, BKCa, Maxi K+ or slo) is part of a structurally diverse group of K+ channels that are activated by an increase in intracellular Ca2+. KCa1.1 shows a large single channel conductance when recorded electrophysiologically and hence its name. It differs from the rest of the subfamily members in that it can be activated by both an increase in intracellular Ca2+ and by membrane depolarization. KCa1.1 is expressed in virtually all cell types where it causes hyperpolarization and helps to connect intracellular Ca2+ signaling pathways and membrane excitability.Indeed, KCa1.1 channels have a crucial role in smooth muscle contractility, neuronal spike shaping and neurotransmitter release.

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1

Short Description :

A Rabbit Polyclonal Antibody to KCNMA1 (KCa1.1) Channel

Long Description :

Anti-KCNMA1 (KCa1.1) (1184-1200) Antibody (#APC-107) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot, immunocytochemistry, immunohistochemistry, and immunoprecipitation applications. It has been designed to recognize KCNMA1 from human, rat, and mouse samples.

Negative Control :

BLP-PC107

Positive Control :

Synonyms :

Large conductance calcium-activated potassium channel subfamily M subunit alpha-1, BKCa alpha, Maxi K+, Slo1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNMA1

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:500

more info or order :

Alomone Labs product webpage