Anti-Kir6.1 (KCNJ8) Antibody | Alomone Labs APC-105 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Kir6.1 (KCNJ8) Antibody

catalog :

APC-105

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 3

Reference
He M, Shi W, Yu M, Li X, Xu J, Zhu J, et al. Nicorandil Attenuates LPS-Induced Acute Lung Injury by Pulmonary Endothelial Cell Protection via NF-κB and MAPK Pathways. Oxid Med Cell Longev. 2019;2019:4957646 pubmed publisher
Gangadhariah M, Dieckmann B, Lantier L, Kang L, Wasserman D, Chiusa M, et al. Cytochrome P450 epoxygenase-derived epoxyeicosatrienoic acids contribute to insulin sensitivity in mice and in humans. Diabetologia. 2017;60:1066-1075 pubmed publisher
Wu Y, He M, Ye J, Ma S, Huang W, Wei Y, et al. Activation of ATP-sensitive potassium channels facilitates the function of human endothelial colony-forming cells via Ca2+ /Akt/eNOS pathway. J Cell Mol Med. 2017;21:609-620 pubmed publisher

image

image 1 :

Western blot analysis of rat heart membranes: - 1. Anti-Kir6.1 (KCNJ8) Antibody (#APC-105), (1:200).2. Anti-Kir6.1 (KCNJ8) Antibody, preincubated with Kir6.1/KCNJ8 Blocking Peptide (#BLP-PC105).

image 2 :

Expression of Kir6.1 in rat uterus - Immunohistochemical stain of pregnant rat longitudinal section of the myometrium using Anti-Kir6.1 (KCNJ8) Antibody (#APC-105), (1:50). Strong and specific staining is evident in smooth muscles cells both in the myometrium (blue arrow) and muscular layers of blood vessels (green arrow). Peroxidase reaction with DAB were used for the color reaction. Hematoxilin is used as the counterstain.

image 3 :

Western blot analysisof rat heart membranes: - 1.Anti-Kir6.1 (KCNJ8) Antibody (#APC-105) (1:200).2. Anti-Kir6.1 (KCNJ8) Antibody preincubated with the control peptide antigen.

product information

CAT :

APC-105

SKU :

APC-105-CF_0.2 ml

Product Name :

Anti-Kir6.1 (KCNJ8) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63664

Applications :

IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC105

Homology :

Mouse, human, pig, rabbit - 15/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

(C)KRNSMRRNNSMRRSN, corresponding to amino acid residues 382-396 of rat Kir6.1

Product Page - Scientific background :

Kir6.1 is a member of the inward rectifier K+ channels (Kir channels), a large family of voltage-independent K+ channels largely involved in stabilization of the membrane resting potential and in K+ transport across membranes.Kir6.1, like its close relative Kir6.2, is highly sensitive to inhibition by intracellular ATP. Closure of the channel leads to membrane depolarization thereby coupling intracellular metabolism to cellular excitability.Kir6.1 presents the common topology of the inward-rectifier superfamily: two transmembrane domains flanking a highly conserved pore region with the N- and C-termini located intracellularly.The functional ATP sensitive channel (KATP) is composed of octamers of four Kir6.x subunits and four members of the sulfonylurea receptor family SUR1, SUR2A and SUR2B.The channel's tissue distribution is relatively broad with expression detected in heart, brain, and smooth muscle.

Applications may also work in :

IF IHC IP WB

Supplier :

Alomone Labs

Target :

ATP-sensitive inward rectifier potassium channel 8, uKATP-1

Short Description :

A Rabbit Polyclonal Antibody to Kir6.1 (KCNJ8) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Kir6.1. Anti-Kir6.1 (KCNJ8) Antibody (#APC-105) can be used in western blot, immunoprecipitation and immunohistochemistry applications. It has been designed to recognize Kir6.1 from human, rat and mouse samples. The antibody is specific for Kir6.1 and does not cross react with Kir6.2.

Negative Control :

BLP-PC105

Positive Control :

Synonyms :

ATP-sensitive inward rectifier potassium channel 8, uKATP-1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNJ8

AB Specifictiy :

The antibody is specific for Kir6.1 and does not cross react with Kir6.2.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:50

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage