Anti-KCNH1 (EAG-1) Antibody | Alomone Labs APC-104 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNH1 (EAG-1) Antibody

catalog :

APC-104

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Fang Y, Fu S, Hsu P, Chang P, Huang J, Chiu Y, et al. Identification of MKRN1 as a second E3 ligase for Eag1 potassium channels reveals regulation via differential degradation. J Biol Chem. 2021;296:100484 pubmed publisher
Peretti M, Badaoui M, Girault A, Van Gulick L, Mabille M, Tebbakha R, et al. Original association of ion transporters mediates the ECM-induced breast cancer cell survival: Kv10.1-Orai1-SPCA2 partnership. Sci Rep. 2019;9:1175 pubmed publisher
Badaoui M, Mimsy Julienne C, Saby C, Van Gulick L, Peretti M, Jeannesson P, et al. Collagen type 1 promotes survival of human breast cancer cells by overexpressing Kv10.1 potassium and Orai1 calcium channels through DDR1-dependent pathway. Oncotarget. 2018;9:24653-24671 pubmed publisher
Hsu P, Chiu Y, Lin T, Jeng C. Ca(2+)-binding protein centrin 4 is a novel binding partner of rat Eag1 K(+) channels. FEBS Open Bio. 2016;6:349-57 pubmed publisher

image

image 1 :

Western blot analysis of rat brain lysate (lanes 1 and 3) and HEK-KCNH1 (lanes 2 and 4): - 1,2. Anti-KCNH1 (EAG-1) Antibody (#APC-104), (1:200).3,4. Anti-KCNH1 (EAG-1) Antibody, preincubated with KCNH1/EAG-1 Blocking Peptide (#BLP-PC104).

image 2 :

Expression of EAG1in human MDA-468 mammary gland adenocarcinoma cells. - Immunocytochemical staining of human MDA-468 mammary gland adenocarcinoma cells. A. Cells were stained withAnti-KCNH1(EAG-1) Antibody(#APC-104) (1:200) followed by goat anti-rabbit-AlexaFluor-555 secondary antibody (red). B.Nuclei were visualized withthe cell-permeable dye Hoechst 33342 (blue). C.Merged imageof panels A and B.

image 3 :

Western blot analysisof rat brain lysate (lanes 1 and 3) and HEK-KCNH1(lanes 2 and 4): - 12.Anti-KCNH1(EAG-1) Antibody(#APC-104) (1:200).34. Anti-KCNH1(EAG-1) Antibody preincubated with the negative control antigen.

product information

CAT :

APC-104

SKU :

APC-104-CF_0.2 ml

Product Name :

Anti-KCNH1 (EAG-1) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q63472

Applications :

IC IF IHC IP WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC104

Homology :

Mouse - identical; human -14/16 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

GDPAKRKGWARFKDAC, corresponding to amino acid residues 802-817 of rat KCNH1

Product Page - Scientific background :

KV10.1 is a member of the Ether-a-go-go family of voltage-activated potassium channels. To date, the family includes eight members: the closely related KV10.2 (EAG-2) protein, the KV12 (elk) (elk1, elk2, elk3) and the KV11 (erg) proteins (erg1, erg2, erg3). As with the other KV superfamily members, KV10.1 shows the characteristic 6 transmembrane domains with intracelullar N- and C-termini.The KV10 subfamily current is characterized by outward rectification without inactivation and slow activation kinetics. Its expression is normally confined to the brain where its physiological function has not yet been clarified.Intriguingly, the KV10.1 channel has been implicated in malignant tumor development. It has been shown that KV10.1 was inappropriately expressed in several cancer cell lines. Moreover, one study showed that KV10.1 by itself had oncogenic potential, as a cell line transfected with the channel, induced aggressive and faster tumor growth in vivo as compared to a cell line transfected with an unrelated KV channel.

Applications may also work in :

IC IF IHC IP WB

Supplier :

Alomone Labs

Target :

KV10.1, Ether-a-go-go potassium channel 1, hEAG1

Short Description :

A Rabbit Polyclonal Antibody to KV10.1 Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an intracellular epitope of the rat KV10.1 channel. Anti-KCNH1 (EAG-1) Antibody (#APC-104) can be used in western blot, immunoprecipitation, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize KV10.1 from rat, human, and mouse samples. The antibody is specific for KV10.1 and will not cross-react with KV10.2.

Negative Control :

BLP-PC104

Positive Control :

Synonyms :

KV10.1, Ether-a-go-go potassium channel 1, hEAG1

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNH1

AB Specifictiy :

The antibody is specific for KV10.1 and will not cross-react with KV10.2.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage