Anti-KCNN3 (KCa2.3, SK3) (C-term) Antibody | Alomone Labs APC-103 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNN3 (KCa2.3, SK3) (C-term) Antibody

catalog :

APC-103

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Zak M, Kestler B, Cornwell T, Taylor M. Augmented KCa2.3 Channel Feedback Regulation of Oxytocin Stimulated Uterine Strips from Nonpregnant Mice. Int J Mol Sci. 2021;22: pubmed publisher
Pinilla E, S xe1 nchez A, Mart xed nez M, Mu xf1 oz M, Garc xed a Sacrist xe1 n A, K xf6 hler R, et al. Endothelial KCa 1.1 and KCa 3.1 channels mediate rat intrarenal artery endothelium-derived hyperpolarization response. Acta Physiol (Oxf). 2021;231:e13598 pubmed publisher
Fan X, Yu Y, Lan H, Ou X, Yang L, Li T, et al. Ca2+/Calmodulin-Dependent Protein Kinase II (CaMKII) Increases Small-Conductance Ca2+-Activated K+ Current in Patients with Chronic Atrial Fibrillation. Med Sci Monit. 2018;24:3011-3023 pubmed publisher
Yang M, Camara A, Aldakkak M, Kwok W, Stowe D. Identity and function of a cardiac mitochondrial small conductance Ca2+-activated K+ channel splice variant. Biochim Biophys Acta Bioenerg. 2017;1858:442-458 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KCNN3 (KCa2.3, SK3) (C-term) Antibody (#APC-103), (1:200).2. Anti-KCNN3 (KCa2.3, SK3) (C-term) Antibody, preincubated with KCNN3/KCa2.3 (C-term) Blocking Peptide (#BLP-PC103).

image 2 :

Western blot analysis of rat brain membranes: - 1. Anti-KCNN3 (KCa2.3 SK3) (C-term) Antibody(#APC-103)(1:200).2. Anti-KCNN3 (KCa2.3 SK3) (C-term) Antibody preincubated with the control peptide antigen.

image 3 :

Peptide (C)KDNGELPN DERFSVD corresponding to amino acid residues 583-597 of rat NrCAM (Accession P97686). Extracellular N-terminus.

product information

CAT :

APC-103

SKU :

APC-103-CF_0.2 ml

Product Name :

Anti-KCNN3 (KCa2.3, SK3) (C-term) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9UGI6

Applications :

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC103

Homology :

Rat, mouse, pig - identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminal domain

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)KQIGSLESKLEHLTAS, corresponding to amino acid residues 659-674 of human KCNN3

Product Page - Scientific background :

KCa2.3 (KCNN3, SK3) is a member of the Ca2+-activated K+ channel family with small conductance that includes KCa2.1 (KCNN1, SK1) and KCa2.2 (KCNN2, SK2). The channel is voltage insensitive and is activated by intracellular Ca2+ in the submicromolar range. It has, though, a similar topology to that of voltage-dependent K+ channels (KV channels), that is six transmembrane domains and intracellular N- and C-termini. The functional channel of all the KCa2 family members is a multimeric protein composed of four pore-forming subunits.KCa2 channels are extremely sensitive to the levels of intracellular Ca2+ and concentrations as low as 300-700 nM can open the channels very rapidly (5-15 ms). Hence, the KCa2 channels are highly sensitive and fast Ca2+ sensors resembling other known Ca2+-binding proteins. This type of Ca2+-dependent activation is achieved by the constitutive binding of the KCa2 channels to calmodulin, a highly expressed Ca2+-binding protein via a calmodulin-binding domain situated at the cytoplasmic C-terminus.Pharmacologically, the KCa2 channels are the only known targets of the bee venom toxin Apamin, with KCa2.1 being the least sensitive, KCa2.2 the most sensitive and KCa2.3 showing intermediate sensitivity.KCa2.3 is predominantly expressed in the nervous system although expression in endothelial cells, heart and liver have been described.KCa2.3 is known to be involved in the regulation of neuronal excitability. They do so mainly via a phenomenon known as after hyperpolarization in which KCa2 channels open in response to increased intracellular Ca2+ concentrations that result from the entry of extracellular Ca2+ through voltage-dependent Ca2+ channels during action potentials. In this way, KCa2 channels effectively form a Ca2+-mediated feedback loop.KCa2.3 is involved in the control of firing rate and subsequent dopamine secretion from midbrain dopaminergic neurons. Since malfunction of these neurons is involved in several pathological disorders such as Parkinson's disease and Schizophrenia, modulators of the KCa2.3 channels have been proposed to be of therapeutic value in these diseases.

Applications may also work in :

Supplier :

Alomone Labs

Target :

Small conductance calcium-activated potassium channel protein 3, SKCa3

Short Description :

A Rabbit Polyclonal Antibody to KCNN3 (KCa2.3, SK3) Channel

Long Description :

Anti-KCNN3 (KCa2.3, SK3) (C-term) Antibody (#APC-103) is a highly specific antibody directed against an epitope of the human KCNN3 channel. The antibody can be used in western blot analysis. It has been designed to recognize KCNN3 from human, rat, and mouse samples.

Negative Control :

BLP-PC103

Positive Control :

Synonyms :

Small conductance calcium-activated potassium channel protein 3, SKCa3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNN3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

IP IHC ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage