Anti-KCNC3 (KV3.3) Antibody | Alomone Labs APC-102 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KCNC3 (KV3.3) Antibody

catalog :

APC-102

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 5

Reference
Richardson A, Ciampani V, Stancu M, Bondarenko K, Newton S, Steinert J, et al. Kv3.3 subunits control presynaptic action potential waveform and neurotransmitter release at a central excitatory synapse. elife. 2022;11: pubmed publisher
Stevens S, Longley C, Ogawa Y, Teliska L, Arumanayagam A, Nair S, et al. Ankyrin-R regulates fast-spiking interneuron excitability through perineuronal nets and Kv3.1b K+ channels. elife. 2021;10: pubmed publisher
Zhang Y, Varela L, Szigeti Buck K, Williams A, Stoiljkovic M, Sestan Pesa M, et al. Cerebellar Kv3.3 potassium channels activate TANK-binding kinase 1 to regulate trafficking of the cell survival protein Hax-1. Nat Commun. 2021;12:1731 pubmed publisher
Housley S, Nardelli P, Carrasco D, Rotterman T, Pfahl E, Matyunina L, et al. Cancer Exacerbates Chemotherapy-Induced Sensory Neuropathy. Cancer Res. 2020;80:2940-2955 pubmed publisher
Mao H, Hamodeh S, Skodras A, Sultan F. Quantitative organization of the excitatory synapses of the primate cerebellar nuclei: further evidence for a specialized architecture underlying the primate cerebellum. Brain Struct Funct. 2019;224:1987-1998 pubmed publisher

image

image 1 :

Western blotting of rat brain membranes: - 1. Anti-KCNC3 (KV3.3) Antibody (#APC-102), (1:200). 2. Anti-KCNC3 (KV3.3) Antibody, preincubated with KCNC3/Kv3.3 Blocking Peptide (#BLP-PC102).

image 2 :

Western blotting of rat brain membranes: - 1.Anti-KCNC3 (KV3.3)Antibody (#APC-102) (1:200). 2.Anti-KCNC3 (KV3.3) Antibody preincubated with the negative control antigen.

image 3 :

Expression of N-Cadherin in mouse heart - Immunohistochemical staining of mouse heart free floating frozen sections using Anti-N-Cadherin-ATTO-594 Antibody (#ANR-082-AR) (1:200). N-Cadherin (red) appears in intercalated discs (arrows). Nuclei are stained with DAPI as the counterstain (blue).

product information

CAT :

APC-102

SKU :

APC-102-CF_0.2 ml

Product Name :

Anti-KCNC3 (KV3.3) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q01956

Applications :

IC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC102

Homology :

Human, mouse-identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Intracellular, C-terminus

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Rat

Sequence :

KSPITPGSRGRYSRDRAC, corresponding to amino acid residues 701-718 of rat KCNC3

Product Page - Scientific background :

KV3.3 is a member of the Shaw-type family of voltage-gated K+ channels that includes four members (KV3.1-3.4). KV3 subfamily members inactivate very rapidly and therefore are thought to play a role in the repolarization of action potentials and to facilitate repetitive high frequency firing. KV3.3 is mostly localized in the brain, although it has been observed in vascular smooth muscle cells and eye epithelium. In the brain its expression pattern largely overlaps that of the KV3.1 channel suggesting that they may form functional heteromers. Indeed, mouse knockouts of both KV3.1 and KV3.3, but not either channel alone, display severe motor defects.

Applications may also work in :

IC IF IHC WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily C member 3, KSHIIID, SCA13

Short Description :

A Rabbit Polyclonal Antibody to KCNC3 (KV3.3) Channel

Long Description :

Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of the rat KV3.3 channel. Anti-KCNC3 (KV3.3) Antibody (#APC-102) can be used in western blot, immunohistochemistry and immunocytochemistry applications. It has been designed to recognize KV3.3 from rat, mouse and human samples.

Negative Control :

BLP-PC102

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily C member 3, KSHIIID, SCA13

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNC3

AB Specifictiy :

Recognizes only splice variant KV3.3-1.

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage