Anti-KV1.3 (KCNA3) (extracellular) Antibody | Alomone Labs APC-101 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV1.3 (KCNA3) (extracellular) Antibody

catalog :

APC-101

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation

more info or order :

Alomone Labs product webpage

citations: 10

Published Application/Species/Sample/Dilution	Reference
western blot; mouse; 1:400; loading ...; fig 4a	Angelova D, Brown D. Model Senescent Microglia Induce Disease Related Changes in α-Synuclein Expression and Activity. Biomolecules. 2018;8: pubmed publisher
	Shi S, Zhao Q, Ke C, Long S, Zhang F, Zhang X, et al. Loureirin B Exerts its Immunosuppressive Effects by Inhibiting STIM1/Orai1 and KV1.3 Channels. Front Pharmacol. 2021;12:685092 pubmed publisher
	Peruzzo R, Mattarei A, Azzolini M, Becker Flegler K, Romio M, Rigoni G, et al. Insight into the mechanism of cytotoxicity of membrane-permeant psoralenic Kv1.3 channel inhibitors by chemical dissection of a novel member of the family. Redox Biol. 2020;37:101705 pubmed publisher
	Nguyen H, Di Lucente J, Chen Y, Cui Y, Ibrahim R, Pennington M, et al. Biophysical basis for Kv1.3 regulation of membrane potential changes induced by P2X4-mediated calcium entry in microglia. Glia. 2020;68:2377-2394 pubmed publisher
	Peruzzo R, Mattarei A, Romio M, Paradisi C, Zoratti M, Szabo I, et al. Regulation of Proliferation by a Mitochondrial Potassium Channel in Pancreatic Ductal Adenocarcinoma Cells. Front Oncol. 2017;7:239 pubmed publisher
	Rangaraju S, Raza S, Pennati A, Deng Q, Dammer E, Duong D, et al. A systems pharmacology-based approach to identify novel Kv1.3 channel-dependent mechanisms in microglial activation. J Neuroinflammation. 2017;14:128 pubmed publisher
	Leanza L, Romio M, Becker K, Azzolini M, Trentin L, ManagÃ² A, et al. Direct Pharmacological Targeting of a Mitochondrial Ion Channel Selectively Kills Tumor Cells InÂ Vivo. Cancer Cell. 2017;31:516-531.e10 pubmed publisher
	Vivekananda U, Novak P, Bello O, Korchev Y, Krishnakumar S, Volynski K, et al. Kv1.1 channelopathy abolishes presynaptic spike width modulation by subthreshold somatic depolarization. Proc Natl Acad Sci U S A. 2017;114:2395-2400 pubmed publisher
	Zaccagnino A, ManagÃ² A, Leanza L, Gontarewitz A, Linder B, Azzolini M, et al. Tumor-reducing effect of the clinically used drug clofazimine in a SCID mouse model of pancreatic ductal adenocarcinoma. Oncotarget. 2017;8:38276-38293 pubmed publisher
	JimÃ©nez PÃ©rez L, Cidad P, Ãlvarez Miguel I, Santos HipÃ³lito A, Torres Merino R, Alonso E, et al. Molecular Determinants of Kv1.3 Potassium Channels-induced Proliferation. J Biol Chem. 2016;291:3569-80 pubmed publisher

image

image 1 :

Western blot analysis of rat brain membranes: - 1. Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101), (1:500). 2. Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).

image 2 :

Peptide KDYPASTSQDSFEA(C) corresponding to amino acid residues 263-276 of human KV1.3 (AccessionP22001). 1stextracellular loop.

product information

CAT :

APC-101

SKU :

APC-101-CF_0.2 ml

Product Name :

Anti-KV1.3 (KCNA3) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P22001

Applications :

IC IF IFC IHC IP LCI WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC101

Homology :

Rat, mouse - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular loop between domains S1 and S2.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3

Product Page - Scientific background :

KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. The prominence of KV1.3 channels in these cells (particularly in T lymphocytes) directed much research attention. It was found that KV1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2 Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.1 The central role of KV1.3 in immune system cells created a real need for a specific antibody that would be able to work in flow cytometry applications.

Applications may also work in :

IC IF IFC IHC IP LCI WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily A member 3

Short Description :

A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel

Long Description :

Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging and indirect flow cytometry applications. It has been designed to recognize KV1.3 potassium channel from human, mouse and rat samples.

Negative Control :

BLP-PC101

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily A member 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNA3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

ICC

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

1:200-1:500

more info or order :

Alomone Labs product webpage