Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody | Alomone Labs APC-101-GP product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody

catalog :

APC-101-GP

clonality :

polyclonal

host :

guinea-pigs

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry

more info or order :

Alomone Labs product webpage

citations: 4

Reference
Chen Y, Cui Y, Singh L, Wulff H. The potassium channel Kv1.3 as a therapeutic target for immunocytoprotection after reperfusion. Ann Clin Transl Neurol. 2021;8:2070-2082 pubmed publisher
Sarkar S, Nguyen H, Malovic E, Luo J, Langley M, Palanisamy B, et al. Kv1.3 modulates neuroinflammation and neurodegeneration in Parkinson's disease. J Clin Invest. 2020;130:4195-4212 pubmed publisher
Lowinus T, Heidel F, Bose T, Nimmagadda S, Schnöder T, Cammann C, et al. Memantine potentiates cytarabine-induced cell death of acute leukemia correlating with inhibition of Kv1.3 potassium channels, AKT and ERK1/2 signaling. Cell Commun Signal. 2019;17:5 pubmed publisher
Grimaldi A, D Alessandro G, Di Castro M, Lauro C, Singh V, Pagani F, et al. Kv1.3 activity perturbs the homeostatic properties of astrocytes in glioma. Sci Rep. 2018;8:7654 pubmed publisher

image

image 1 :

Western blot analysis of mouse (lanes 1 and 3) and rat (lanes 2 and 4) brain lysates: - 1,2. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP), (1:200).3,4. Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).

image 2 :

Expression of KV1.3 in mouse cerebellum - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections using Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP), (1:100). KV1.3 staining (red) is detected in Bergmann glia outlines (arrow). DAPI is used as the counterstain (blue) .

product information

CAT :

APC-101-GP

SKU :

APC-101-GP-CF_0.2 ml

Product Name :

Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P22001

Applications :

IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Guinea Pig

Blocking Peptide :

BLP-PC101

Homology :

Rat, mouse - 12/14 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Guinea pig total IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Extracellular loop between domains S1 and S2.

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3

Product Page - Scientific background :

KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. The prominence of KV1.3 channels in these cells (particularly in T lymphocytes) directed much research attention. It was found that KV1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2 Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.1

Applications may also work in :

IF IHC WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily A member 3

Short Description :

A Guinea Pig Polyclonal Antibody to KV1.3 (KCNA3) Channel

Long Description :

Guinea pig Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101-GP, formerly AGP-005) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize KV1.3 potassium channel from mouse, rat, and human samples. The antigen used to immunize guinea pigs is the same as Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Negative Control :

BLP-PC101

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily A member 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNA3

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:100

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage