Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody | Alomone Labs APC-101-F product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody

catalog :

APC-101-F

clonality :

polyclonal

host :

domestic rabbit

conjugate :

FITC

clone name :

reactivity :

human, mouse, rat

application :

immunocytochemistry, flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Lowinus T, Heidel F, Bose T, Nimmagadda S, Schnöder T, Cammann C, et al. Memantine potentiates cytarabine-induced cell death of acute leukemia correlating with inhibition of Kv1.3 potassium channels, AKT and ERK1/2 signaling. Cell Commun Signal. 2019;17:5 pubmed publisher

image

image 1 :

Cell surface detection of KV1.3 in live intact Jurkat (human T cell leukemia) cells: Cell surface detection of KV1.3 in live intact Jurkat (human T cell leukemia) cells: Red line: Unstained cells. Green line: Cells + Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody (#APC-101-F), (0.5-5 µg antibody / 1x106 cells).

product information

CAT :

APC-101-F

SKU :

APC-101-F_15 mcl

Product Name :

Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P22001

Applications :

FC IC IF LCI

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC101

Homology :

Rat, mouse - 12/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

15 µl or 50 µl double distilled water (DDW), depending on the sample size.

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular loop between domains S1 and S2.

Label :

Fluorescein isothiocyanate (FITC).

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3

Product Page - Scientific background :

KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. The prominence of KV1.3 channels in these cells (particularly in T lymphocytes) directed much research attention. It was found that KV1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2 Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.1KV1.3 channels are potently inhibited by several venomous peptide toxins among them Charybdotoxin (2.6 nM), Noxiustoxin (1 nM), Kaliotoxin (0.65 nM), Margatoxin (0.05 nM), Agitoxin-1 (1.7 nM), Agitoxin-2 (0.004 nM), Hongotoxin-1 (0.09 nM) and Stichodactyla toxin (0.01 nM).3-7The central role of KV1.3 in immune system cells created a real need for a specific antibody that would be able to work in flow cytometry applications.

Applications may also work in :

FC IC IF LCI

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily A member 3

Short Description :

A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel Conjugated to the Fluorescent Dye FITC

Long Description :

Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize KV1.3 potassium channel from human, mouse, and rat samples. Anti-KV1.3 (KCNA3) (extracellular)-FITC Antibody (#APC-101-F) is directly conjugated to fluorescein isothiocyanate (FITC). This labeled antibody can be used in immunofluorescent applications such as direct flow cytometry and live cell imaging.

Negative Control :

RIC-001-F

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily A member 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNA3

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Cited Application :

Clone :

Standard quality control of each lot :

Western blot analysis (unlabeled antibody, #APC-101), and direct flow cytometry (labeled antibody).

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

Contact Alomone for information

Application Dilutions: Western blot wb :

Contact Alomone for information

more info or order :

Alomone Labs product webpage