Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody | Alomone Labs APC-101-B product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody

catalog :

APC-101-B

clonality :

polyclonal

host :

domestic rabbit

conjugate :

biotin

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Bednenko J, Harriman R, Mariën L, Nguyen H, Agrawal A, Papoyan A, et al. A multiplatform strategy for the discovery of conventional monoclonal antibodies that inhibit the voltage-gated potassium channel Kv1.3. MAbs. 2018;10:636-650 pubmed publisher

image

image 1 :

Western blot analysis of human Jurkat T cell leukemia cell lysate (lanes 1 and 3) and mouse brain lysate (lanes 2 and 4): - 1-2. Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (#APC-101-B), (1:1000).3-4. Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody, preincubated with Kv1.3/KCNA3 (extracellular) Blocking Peptide (#BLP-PC101).

image 2 :

Multiplex staining of KV1.3 and KV1.5 in mouse cerebellum - Immunohistochemical staining of mouse perfusion-fixed frozen brain sections using Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (#APC-101-B) (1:400), and Anti-KV1.5 (KCNA5)-ATTO Fluor-550 Antibody (#APC-004-AO), (1:60). A. KV1.3 staining (green) is detected in Bergmann glia soma and processes in the molecular layer (Mol) (arrow). B. Same section shows staining for KV1.5 (red). C. Merge of the two images suggests considerable co-localization in the soma of Bergmann glia. In the molecular layer, the distribution of KV1.5 is diffuse, unlike the discrete staining for KV1.3 in glial processes. Cell nuclei were visualized with DAPI (blue).

image 3 :

Cell surface detection of KV1.3 in live intact human Jurkat T cell leukemia cell line: Cell surface detection of KV1.3 in live intact human Jurkat T cell leukemia cell line: Black line: Cells + Streptavidin-Alexa647. Black line: Cells + Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (#APC-101-B), (1:10) + Streptavidin-Alexa647.

product information

CAT :

APC-101-B

SKU :

APC-101-B_50 mcl

Product Name :

Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

P22001

Applications :

FC IF IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC101

Homology :

Rat, mouse - 12/14 amino acid residues identical

Formulation :

PBS pH7.4, 1% BSA with 0.05% sodium azide

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

50 µl double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

1% BSA, 0.05% NaN3

Immunogen Location :

Extracellular loop between domains S1 and S2.

Label :

Biotin

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

KDYPASTSQDSFEA(C), corresponding to amino acid residues 263-276 of human KV1.3

Product Page - Scientific background :

KV1.3 belongs to the Shaker family of voltage-dependent K+ channels. The channel, encoded by KCNA3, is widely expressed in the brain, lung and osteoclasts and in several cell populations of hematopoietic origin. The prominence of KV1.3 channels in these cells (particularly in T lymphocytes) directed much research attention. It was found that KV1.3 is the main channel responsible for maintaining the resting potential in quiescent cells and regulating the Ca2+ signaling that is indispensable for normal T lymphocyte activation.1,2 Based on the central role of KV1.3 in regulating the initiation of an immune response, the channel has been recognized as a potential target for immunosuppressant drugs.1 The central role of KV1.3 in immune system cells created a real need for a specific antibody that would be able to work in flow cytometry applications.

Applications may also work in :

FC IF IHC WB

Supplier :

Alomone Labs

Target :

Potassium voltage-gated channel subfamily A member 3

Short Description :

A Rabbit Polyclonal Antibody to KV1.3 (KCNA3) Channel Conjugated to Biotin

Long Description :

Anti-KV1.3 (KCNA3) (extracellular) Antibody (#APC-101) is a highly specific antibody directed against an extracellular epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, live cell imaging, and indirect flow cytometry applications. It has been designed to recognize KV1.3 potassium channel from human, mouse, and rat samples. Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody (#APC-101-B) is directly labeled with biotin. Strepavidin tagged with HRP or with a fluorescent probe can then be used to detect the protein. The biotin/strepavidin system is ideal for minimizing cross-reactivity when same species antibodies are simultaneously used. Anti-KV1.3 (KCNA3) (extracellular)-Biotin Antibody has been tested in western blot, immunohistochemistry and direct flow cytometry and is specially suited to experiments requiring simultaneous labeling of different markers.

Negative Control :

BLP-PC101

Positive Control :

Synonyms :

Potassium voltage-gated channel subfamily A member 3

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

KCNA3

Form :

Lyophilized powder

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:400

Application Dilutions: Western blot wb :

1:1000

more info or order :

Alomone Labs product webpage