Anti-Piezo2 Antibody | Alomone Labs APC-090 product information

product summary

company name :

Alomone Labs

product type :

antibody

product name :

Anti-Piezo2 Antibody

catalog :

APC-090

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

Alomone Labs product webpage

citations: 1

Reference
Zeidler M, Kummer K, Schöpf C, Kalpachidou T, Kern G, Cader M, et al. NOCICEPTRA: Gene and microRNA Signatures and Their Trajectories Characterizing Human iPSC-Derived Nociceptor Maturation. Adv Sci (Weinh). 2021;8:e2102354 pubmed publisher

image

image 1 :

Western blot analysis of rat dorsal root ganglion lysate (lanes 1 and 3) and mouse lung lysate (lanes 2 and 4): - 1, 2. Anti-Piezo2 Antibody (#APC-090), (1:200). 3, 4. Anti-Piezo2 Antibody, preincubated with Piezo2 Blocking Peptide (#BLP-PC090).

image 2 :

Expression of Piezo2 in rat dorsal root ganglion. - Immunohistochemical staining of perfusion-fixed frozen rat dorsal root ganglion (DRG) sections with Anti-Piezo2 Antibody (#APC-090), (1:200), followed by goat anti-rabbit-AlexaFluor-488. A. Piezo2 immunoreactivity (green) appeared in neuron profiles (arrows). B. Pre-incubation of the antibody with Piezo2 Blocking Peptide (#BLP-PC090), suppressed staining. Cell nuclei are stained with DAPI (blue).

product information

CAT :

APC-090

SKU :

APC-090-CF_0.2 ml

Product Name :

Anti-Piezo2 Antibody

Group Type :

Antibodies

Product Type :

Antibodies

Clonality :

Polyclonal

Accession :

Q9H5I5

Applications :

IHC WB

Reactivity :

Human Rat Mouse

Host :

Rabbit

Blocking Peptide :

BLP-PC090

Homology :

Mouse, rat - 12/13 amino acid residues identical

Formulation :

PBS pH7.4

isotype :

Rabbit IgG

Peptide confirmation :

Confirmed by amino acid analysis and mass spectrometry

Reconstitution :

0.2 ml double distilled water (DDW).

Antibody Concentration After Reconstitut ... :

1 mg/ml

Storage After Reconstitution :

The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Preservative :

No Preservative

Immunogen Location :

Located in an internal region of human Piezo2 (the exact topology of Piezo2 remains to be defined).

Label :

Unconjugated

Storage Before Reconstitution :

The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Shipping and storage :

Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C

immunogen source species :

Human

Sequence :

(C)RTIFHDITRLHLD, corresponding to amino acid residues 1092 - 1104 of human Piezo2

Product Page - Scientific background :

Piezo-type mechanosensitive ion channel component 2 (Piezo2) is a homotrimeric ion channel. Piezo2 depicts rapidly adapting mechanically activated (MA) currents in somatosensory neurons.Piezo1 and Piezo2 comprise the Piezo protein family. These are large transmembrane proteins are conserved among various species, and all have between 24 and 36 predicted transmembrane domains. Both channels open in response to mechanical stimuli, allowing positively charged ions, including calcium, to flow into the cell1.Piezo2 protein contains over 2,800 residues; it forms a three-bladed, propeller like trimer that comprises 114 transmembrane helices (38 per protomer). Transmembrane helices 1-36 (TM1-36) are folded into nine tandem units of four transmembrane helices each to form the unusual non-planar blades2.Piezo2 has an essential role in sensing gentle touch, tactile pain, airway stretch and lung inflation. It also serves as a baroreceptor for sensing blood pressure and regulating heart rate. Mutations in human Piezo2 are associated with distal arthrogryposis, a disorder characterized by joint deformities (contractures) that restrict movement in the hands and feet3,4.

Applications may also work in :

IHC WB

Supplier :

Alomone Labs

Target :

Piezo-Type Mechanosensitive Ion Channel Component 2, Protein FAM38B

Short Description :

A Rabbit Polyclonal Antibody to Piezo2

Long Description :

Anti-Piezo2 Antibody (#APC-090) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize Piezo2 from human, rat, and mouse samples.

Negative Control :

BLP-PC090

Positive Control :

Synonyms :

Piezo-Type Mechanosensitive Ion Channel Component 2, Protein FAM38B

Lead Time :

1-2 Business Days

Country of origin :

Israel/IL

Applications key :

CBE- Cell-based ELISA, FC- Flow cytometry, ICC- Immunocytochemistry, IE- Indirect ELISA, IF- Immunofluorescence, IFC- Indirect flow cytometry, IHC- Immunohistochemistry, IP- Immunoprecipitation, LCI- Live cell imaging, N- Neutralization, WB- Western blot

Specifictiy :

PIEZO2

Form :

Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.

Comment :

Contact Alomone Labs for technical support and product customization

Species reactivity key :

H- Human, M- Mouse, R- Rat

Is Toxin :

Purity :

Affinity purified on immobilized antigen.

UNSPSC :

41116161

KO-Validated :

yes

Cited Application :

IP IHC ICC Whole Tissue Staining

Clone :

Standard quality control of each lot :

Western blot analysis

Antigen preadsorption control :

1 µg peptide per 1 µg antibody

Application Dilutions: Immunohistochemis ... :

1:200

Application Dilutions: Western blot wb :

1:200

more info or order :

Alomone Labs product webpage